Quantitative polymerase chain reaction using photothermal conversion of Au nanoshell for point-of-care diagnostics

The global rise of infectious diseases underscores the urgent need for rapid, accurate diagnostics. Our study introduces a novel polymerase chain reaction technique utilizing gold nanoshells for faster and more efficient DNA amplification. These nanoshells' unique properties enable rapid heating in the near-infrared spectrum, accelerating the PCR process by quickly reaching optimal temperatures. This approach streamlines DNA amplification and ensures detection of small DNA quantities. Integrating photothermal PCR with advanced real-time fluorometry and non-invasive temperature monitoring, we can amplify DNA in just 25 minutes and detect as little as 50 picograms. The use of gold nanoshells' heating capabilities leads to quicker, more sensitive DNA detection. This innovation is a significant advancement in PCR technology, especially for point-of-care diagnostics, promising quick identification of DNA markers vital for prompt infectious disease diagnosis. It's particularly valuable in urgent care or resource-limited settings, where rapid, reliable results are essential.