B cells in the pneumococcus-infected lung are heterogeneous and require CD4+ T cell help including CD40L to become resident memory B cells

被引：2

作者：

Etesami, Neelou S. ^{[1
,2
]}

Barker, Kimberly A. ^{[1
,2
]}

Shenoy, Anukul T. ^{[1
,3
]}

De Ana, Carolina Lyon ^{[1
,2
]}

Arafa, Emad I. ^{[1
]}

Grifno, Gabrielle N. ^{[4
]}

Matschulat, Adeline M. ^{[1
,5
]}

Vannini, Michael E. ^{[1
]}

Pihl, Riley M. F. ^{[1
]}

Breen, Michael P. ^{[1
]}

Soucy, Alicia M. ^{[1
]}

Goltry, Wesley N. ^{[1
]}

Ha, Catherine T. ^{[1
]}

Betsuyaku, Hanae ^{[1
]}

Browning, Jeffrey L. ^{[2
]}

Varelas, Xaralabos ^{[1
,5
]}

Traber, Katrina E. ^{[1
,6
]}

Jones, Matthew R. ^{[1
,6
]}

Quinton, Lee J. ^{[1
,2
,6
,7
,8
]}

Maglione, Paul J. ^{[1
,2
,6
]}

Nia, Hadi T. ^{[1
,4
]}

Belkina, Anna C. ^{[1
,8
,9
]}

Mizgerd, Joseph P. ^{[1
,2
,5
,6
]}

机构：

[1] Boston Univ, Chobanian & Avedisian Sch Med, Pulm Ctr, Boston, MA 02118 USA

[2] Boston Univ, Chobanian & Avedisian Sch Med, Dept Virol Immunol & Microbiol, Boston, MA 02118 USA

[3] Univ Michigan, Med Sch, Dept Microbiol & Immunol, Ann Arbor, MI USA

[4] Boston Univ, Coll Engn, Dept Biomed Engn, Boston, MA USA

[5] Boston Univ, Chobanian & Avedisian Sch Med, Dept Biochem & Cell Biol, Boston, MA 02115 USA

[6] Boston Univ, Dept Med, Chobanian & Avedisian Sch Med, Boston, MA 02118 USA

[7] Univ Massachusetts, Chan Med Sch, Div Infect Dis & Immunol, Worcester, MA USA

[8] Boston Univ, Chobanian & Avedisian Sch Med, Dept Pathol & Lab Med, Boston, MA USA

[9] Boston Univ, Chobanian & Avedisian Sch Med, Flow Cytometry Core Facil, Boston, MA USA

来源：

FRONTIERS IN IMMUNOLOGY | 2024年 / 15卷

关键词：

mucosal immunity; lung immunology; pneumonia; adaptive immunity; B cells; LYMPHOID-TISSUE IBALT; PROTECTION; COLONIZATION; CD69; MECHANISM; CHEMOKINE; SUBSETS; DISEASE; CXCL13; MICE;

D O I：

10.3389/fimmu.2024.1382638

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recovery from respiratory pneumococcal infections generates lung-localized protection against heterotypic bacteria, mediated by resident memory lymphocytes. Optimal protection in mice requires re-exposure to pneumococcus within days of initial infection. Serial surface marker phenotyping of B cell populations in a model of pneumococcal heterotypic immunity revealed that bacterial re-exposure stimulates the immediate accumulation of dynamic and heterogeneous populations of B cells in the lung, and is essential for the establishment of lung resident memory B (B-RM) cells. The B cells in the early wave were activated, proliferating locally, and associated with both CD4(+ )T cells and CXCL13. Antagonist- and antibody-mediated interventions were implemented during this early timeframe to demonstrate that lymphocyte recirculation, CD4(+) cells, and CD40 ligand (CD40L) signaling were all needed for lung B-RM cell establishment, whereas CXCL13 signaling was not. While most prominent as aggregates in the loose connective tissue of bronchovascular bundles, morphometry and live lung imaging analyses showed that lung B-RM cells were equally numerous as single cells dispersed throughout the alveolar septae. We propose that CD40L signaling from antigen-stimulated CD4(+) T cells in the infected lung is critical to establishment of local B-RM cells, which subsequently protect the airways and parenchyma against future potential infections.

引用

页数：20