The role of transforming growth factor-β (TGF-β1) in postmenopausal women with pelvic organ prolapse: An immunohistochemical study

被引：8
作者：
Carlin, Greta Lisa [1 ]
Bodner, Klaus [1 ]
Kimberger, Oliver [2 ]
Haslinger, Peter [3 ]
Schneeberger, Christian [3 ]
Horvat, Reinhard [4 ]
Koelbl, Heinz [1 ]
Umek, Wolfgang [1 ,5 ]
Bodner-Adler, Barbara [1 ,5 ]
机构：
[1] Med Univ Vienna, Dept Gen Gynecol & Gynecol Oncol, Vienna, Austria
[2] Med Univ Vienna, Dept Anesthesiol, Vienna, Austria
[3] Med Univ Vienna, Dept Obstet & Gynecol, Vienna, Austria
[4] Med Univ Vienna, Inst Pathol, Vienna, Austria
[5] Karl Landsteiner Inst Specialised Obstet & Gyneco, Vienna, Austria
来源：
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY-X | 2020年 / 7卷
关键词：
Pelvic organ prolapse; TGF-beta; 1; expression; Immunohistochemistry; Postmenopausal women; Uterosacral ligament; UTEROSACRAL LIGAMENTS; CONNECTIVE-TISSUE; FLOOR DISORDERS; COLLAGEN; EXPRESSION; METABOLISM; SYMPTOMS; STANDARDIZATION; TERMINOLOGY; SUPPORT;
D O I：
10.1016/j.eurox.2020.100111
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
Objective: Aim of the study was to investigate the expression of transforming growth factor-beta 1 (TGF-beta 1), a key regulator of the extracellular matrix composition, in the uterosacral ligaments (USLs) of women with pelvic organ prolapse (POP) compared with controls. We hypothesized that the expression pattern of TGF-beta 1 differs between postmenopausal women with or without POP. Methods: Under ethical approval, USL samples were obtained from postmenopausal women undergoing vaginal hysterectomy for stage two or greater pelvic organ prolapse (cases, n = 70) and from postmenopausal women without pelvic organ prolapse undergoing vaginal hysterectomy for benign indications (controls, n = 30). Immunohistochemical staining was performed from paraffin embedded tissue using anti-TGF-beta 1 antibodies. The expression of TGF-beta 1 was evaluated by the pathologist, who was blinded to all clinical data. Results: The expression of TGF-beta 1 was similar in patients with symptomatic POP (89 % positive) and in controls (90 % positive) without any signs of prolapse (p = 0.091). Age-adjusted analysis did not significantly alter these results. Regarding POP-Q stages, TGF-beta 1 was significantly more frequently expressed in severe prolapse cases compared to moderate/mild cases (POP-Q stage IV versus POP-Q stage II and III; p = 0.001). No significant association could be detected between TGF-beta 1 expression and age, BMI and parity in cases with POP (p > 0.05). As published previously, advanced patients' age as well as early menopausal age remained independent risk factors associated with POP in multiple logistic regression analysis (p = 0.001; p = 0.02). Conclusion: Although our study detected POP-Q stage related alterations in USL composition and TGF-beta 1 expression, there was no significant difference in the expression of TGF-beta 1 in cases with or without prolapse. (c) 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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页数：5
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