High-level expression of xyloglucanase B from Rhizomucor miehei and its application in the preparation of partially hydrolyzed apple pomace xyloglucan

被引：3
作者：
Wang, Nan-nan [1 ]
Li, Yan-xiao [2 ]
Zhu, Chun-hua [1 ]
Yan, Qiao-juan [2 ]
Shi, Ran [1 ]
Jiang, Zheng-qiang [1 ]
机构：
[1] China Agr Univ, Coll Food Sci & Nutr Engn, Key Lab Food Bioengn China Natl Light Ind, 17 Qinghua Donglu, Beijing 100083, Peoples R China
[2] China Agr Univ, Coll Engn, Bioresource Utilizat Lab, Beijing, Peoples R China
来源：
FOOD BIOENGINEERING | 2022年 / 1卷 / 02期
基金：
中国国家自然科学基金;
关键词：
apple pomace; partially hydrolyzed apple pomace xyloglucan; Pichia pastoris; Rhizomucor miehei; xyloglucanase; PICHIA-PASTORIS; ASPERGILLUS-NIGER; PURIFICATION; EXTRACTION; HYDROLASE;
D O I：
10.1002/fbe2.12012
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A xyloglucanase gene (RmXEG12B) was cloned from Rhizomucor miehei CAU432 and successfully expressed in Pichia pastoris. The highest xyloglucanase activity of 26,200U/mL was achieved after 168h high-cell-density fermentation. The optimal pH and temperature of RmXEG12B were 5.0 and 55 degrees C, respectively. RmXEG12B showed good stability within pH of 3.0-10.0 and was stable up to 50 degrees C. It exhibited the highest specific activity (5989.3U/mg) towards tamarind xyloglucan. RmXEG12B hydrolyzed apple pomace xyloglucan to produce the partially hydrolyzed apple pomace xyloglucan, which demonstrated a weight average molecular weight of 1.6x10(4)Da and contained three major oligosaccharides with a degree of polymerization of 7-10. This is a strategy for the comprehensive utilization of apple pomace to produce the partially hydrolyzed apple pomace xyloglucan.
引用
收藏
页码：119 / 125
页数：7
相关论文
共 31 条
[1]   New Family of Carbohydrate-Binding Modules Defined by a Galactosyl-Binding Protein Module from a Cellvibrio japonicus Endo-Xyloglucanase [J].
Attia, Mohamed A. ;
Brumer, Harry .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2021, 87 (05) :1-11
[2]   Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein [J].
Berezina, Oksana V. ;
Herlet, Jonathan ;
Rykov, Sergey V. ;
Kornberger, Petra ;
Zavyalov, Artem ;
Kozlov, Dmitriy ;
Sakhibgaraeva, Liliya ;
Krestyanova, Irina ;
Schwarz, Wolfgang H. ;
Zverlov, Vladimir V. ;
Liebl, Wolfgang ;
Yarotsky, Sergey V. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (14) :5653-5666
[3]   Characterization of an alkali-stable xyloglucanase/mixed-linkage β-glucanase Pgl5A from Paenibacillus sp. S09 [J].
Cheng, Rui ;
Cheng, Long ;
Wang, Lei ;
Fu, Renjie ;
Sun, Xiaqing ;
Li, Jing ;
Wang, Shiming ;
Zhang, Jianfa .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2019, 140 :1158-1166
[4]   Ultrasound-assisted extraction of xyloglucan from apple pomace [J].
Fu, Caili ;
Tian, Haijun ;
Li, Quanhong ;
Cai, Tongyi ;
Du, Wenjuan .
ULTRASONICS SONOCHEMISTRY, 2006, 13 (06) :511-516
[5]   Variability of cell wall polysaccharides composition and hemicellulose enzymatic profile in an apple progeny [J].
Galvez-Lopez, D. ;
Laurens, F. ;
Quemener, B. ;
Lahaye, M. .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2011, 49 (05) :1104-1109
[6]   Expression and characterization of a novel β-glucosidase, with transglycosylation and exo-β-1,3-glucanase activities, from Rhizomucor miehei [J].
Guo, Yu ;
Yan, Qiaojuan ;
Yang, Yu ;
Yang, Shaoqing ;
Liu, Yu ;
Jiang, Zhengqiang .
FOOD CHEMISTRY, 2015, 175 :431-438
[7]   The synergistic action of accessory enzymes enhances the hydrolytic potential of a "cellulase mixture" but is highly substrate specific [J].
Hu, Jinguang ;
Arantes, Valdeir ;
Pribowo, Amadeus ;
Saddler, Jack N. .
BIOTECHNOLOGY FOR BIOFUELS, 2013, 6
[8]   High level expression of a novel β-mannanase from Chaetomium sp exhibiting efficient mannan hydrolysis [J].
Katrolia, Priti ;
Zhou, Peng ;
Zhang, Pan ;
Yan, Qiaojuan ;
Li, Yinan ;
Jiang, Zhengqiang ;
Xu, Haibo .
CARBOHYDRATE POLYMERS, 2012, 87 (01) :480-490
[9]   High level expression of β-mannanase (RmMan5A) in Pichia pastoris for partially hydrolyzed guar gum production [J].
Li, Yan-xiao ;
Yi, Ping ;
Wang, Nan-nan ;
Liu, Jun ;
Liu, Xue-qiang ;
Yan, Qiao-juan ;
Jiang, Zheng-qiang .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 105 :1171-1179
[10]   Molecular Characterization of Xyloglucanase cel74a from Trichoderma reesei [J].
Lopes, Douglas Christian Borges ;
Carraro, Claudia Batista ;
Silva, Roberto Nascimento ;
de Paula, Renato Graciano .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (09)
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