Comparative Cytotoxicity of Menthol and Eucalyptol: An In Vitro Study on Human Gingival Fibroblasts

被引:4
作者
Puig-Herreros, Clara [1 ]
Sanz, Jose Luis [2 ]
Garcia-Bernal, David [3 ,4 ]
Rodriguez-Lozano, Francisco Javier [4 ,5 ]
Murcia, Laura [6 ]
Forner, Leopoldo [2 ]
Ghilotti, James [2 ]
Onate-Sanchez, Ricardo E. [5 ]
Lopez-Garcia, Sergio [2 ]
机构
[1] Univ Valencia, Speech Therapy Univ Clin, Dept Basic Psychol, Valencia 46010, Spain
[2] Univ Valencia, Dept Estomatol, Fac Med & Odontol, Valencia 46010, Spain
[3] Univ Murcia, Fac Med, Dept Biochem & Mol Biol B & Immunol, Murcia 30120, Spain
[4] Biomed Res Inst IMIB, Murcia 30120, Spain
[5] Univ Murcia, Morales Meseguer Hosp, Fac Med, Dept Dermatol Stomatol Radiol & Phys Med, Murcia 30008, Spain
[6] Catholic Univ San Antonio Murcia, Dept Hlth Sci, Murcia 30107, Spain
关键词
eucalyptol; menthol; cytotoxicity; gingival fibroblasts; CELLS; CAMPHOR;
D O I
10.3390/pharmaceutics16040521
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The aim of this study was to assess the influence of eucalyptol and menthol on the cell viability, migration, and reactive oxygen species production of human gingival fibroblasts (GFs) in vitro. Three different concentrations of eucalyptol and menthol were prepared following ISO 10993-5 guidelines (1, 5, and 10 mM). GFs were isolated from extracted teeth from healthy donors. The following parameters were assessed: cell viability via MTT, Annexin-V-FITC and 7-AAD staining, and IC50 assays; cell migration via horizontal scratch wound assay; and cell oxidative stress via reactive oxygen species assay. Data were analyzed using one-way ANOVA and Tukey's post hoc test. Statistical significance was established at p < 0.05. Eucalyptol and Menthol exhibited high cytotoxicity on gingival fibroblasts, as evidenced by cytotoxicity assays. Eucalyptol showed lower levels of cytotoxicity than menthol, compared to the control group. The cytotoxicity of the tested substances increased in a concentration-dependent manner. The same occurred in a time-dependent manner, although even 10 min of exposure to the tested substances showed a high cytotoxicity to the GFs. Commercially available products for oral application with these substances in their composition should be tested for cytotoxicity before their use.
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页数:13
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