Microplastics role in cell migration and distribution during cancer cell division

被引：39

作者：

Brynzak-Schreiber E. ^{[1
]}

Schögl E. ^{[1
,2
]}

Bapp C. ^{[1
]}

Cseh K. ^{[3
]}

Kopatz V. ^{[4
,5
,6
,7
]}

Jakupec M.A. ^{[3
]}

Weber A. ^{[2
]}

Lange T. ^{[8
,9
,10
]}

Toca-Herrera J.L. ^{[2
]}

del Favero G. ^{[11
,12
]}

Wadsak W. ^{[5
,13
]}

Kenner L. ^{[4
,5
,7
,14
,15
]}

Pichler V. ^{[1
,5
]}

机构：

[1] University of Vienna, Department of Pharmaceutical Sciences, Division of Pharmaceutical Chemistry, Vienna

[2] University of Natural Resources and Life Sciences Vienna (BOKU), Department of Bionanosciences, Institute of Biophysics, Vienna

[3] University of Vienna, Faculty of Chemistry, Institute of Inorganic Chemistry, Vienna

[4] Medical University of Vienna, Clinical Institute of Pathology, Department for Experimental and Laboratory Animal Pathology, Vienna

[5] CBmed GmbH – Center for Biomarker Research in Medicine, Styria, Graz

[6] Department for Radiation Oncology, Medical University of Vienna, Vienna

[7] Comprehensive Cancer Center, Medical University Vienna, Vienna

[8] Institute of Anatomy and Experimental Morphology, University Medical Center Hamburg-Eppendorf, Hamburg

[9] Institute of Anatomy I, Jena University Hospital, Jena

[10] University of Vienna, Faculty of Chemistry, Department of Food Chemistry and Toxicology, Vienna

[11] University of Vienna, Faculty of Chemistry, Core Facility Multimodal Imaging, Vienna

[12] Medical University of Vienna, Department of Biomedical Imaging and Image-guided Therapy, Division of Nuclear Medicine, Vienna

[13] Unit of Laboratory Animal Pathology, University of Veterinary Medicine Vienna, Vienna

[14] Christian Doppler Laboratory for Applied Metabolomics, Medical University Vienna, Vienna

来源：

Chemosphere | 2024年 / 353卷

基金：

欧盟地平线“2020”; 奥地利科学基金会;

关键词：

Cell division; Cell migration; Microplastic; Polystyrene;

D O I：

10.1016/j.chemosphere.2024.141463

中图分类号：

学科分类号：

摘要：

Amidst the global plastic pollution crisis, the gastrointestinal tract serves as the primary entry point for daily exposure to micro- and nanoplastics. We investigated the complex dynamics between polystyrene micro- and nanoplastics (PS-MNPs) and four distinct human colorectal cancer cell lines (HT29, HCT116, SW480, and SW620). Our findings revealed a significant size- and concentration dependent uptake of 0.25, 1, and 10 μm PS-MNPs across all cell lines, with HCT116 cells exhibiting the highest uptake rates. During cell division, particles were distributed between mother and daughter cells. Interestingly, we observed no signs of elimination from the cells. Short-term exposure to 0.25 μm particles significantly amplified cell migration, potentially leading to pro-metastatic effects. Particles demonstrated high persistence in 2D and 3D cultures, and accumulation in non-proliferating parts of spheroids, without interfering with cell proliferation or division. Our study unveils the disturbing fact of the persistence and bioaccumulation of MNPs in colorectal cancer cell lines, key toxicological traits under REACH (Regulation concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals). Our observations underscore the potential of MNPs as hidden catalysts for tumor progression, particularly through enhancing cell migration and possibly fueling metastasis - a finding that sheds light on a significant and previously underexplored area of concern. © 2024 The Authors

引用

共 54 条

[1]

Akhatova F., Ishmukhametov I., Fakhrullina G., Fakhrullin R., Nanomechanical atomic force microscopy to probe cellular microplastics uptake and distribution, Int. J. Mol. Sci., 23, 2, (2022)

[2]

Annangi B., Villacorta A., Vela L., Tavakolpournegari A., Marcos R., Hernandez A., Effects of true-to-life PET nanoplastics using primary human nasal epithelial cells, Environ. Toxicol. Pharmacol., 100, (2023)

[3]

Armistead F.J., Gala De Pablo J., Gadelha H., Peyman S.A., Evans S.D., Physical biomarkers of disease progression: on-chip monitoring of changes in mechanobiology of colorectal cancer cells, Sci. Rep., 10, pp. 1-10, (2020)

[4]

Banerjee A., Billey L.O., Shelver W.L., Uptake and toxicity of polystyrene micro/nanoplastics in gastric cells: effects of particle size and surface functionalization, PLoS One, pp. 1-25, (2021)

[5]

Barguilla I., Domenech J., Ballesteros S., Rubio L., Marcos R., Hernandez A., Long-term exposure to nanoplastics alters molecular and functional traits related to the carcinogenic process, J.l Hazard. Mater., (2022)

[6]

Bhattacharjee S., DLS and zeta potential - what they are and what they are not?, J. Contol. Release, pp. 337-351, (2016)

[7]

Brachner A., Fragouli D., Duarte I.F., Farias P.M.A., Dembski S., Ghosh M., Barisic I., Zdzieblo D., Vanoirbeek J., Schwabl P., Neuhaus W., Assessment of human health risks posed by nano-and microplastics is currently not feasible, Int. J. Environ. Res. Public Health, pp. 1-10, (2020)

[8]

Brill-Karniely Y., Dror D., Duanis-Assaf T., Goldstein Y., Schwob O., Millo T., Orehov N., Stern T., Jaber M., Loyfer N., Vosk-Artzi M., Benyamini H., Bielenberg D., Kaplan T., Buganim Y., Reches M., Benny O., Triangular correlation (TrC) between cancer aggressiveness, cell uptake capability, and cell deformability, Sci. Adv., (2020)

[9]

Bruinink A., Wang J., Wick P., Effect of particle agglomeration in nanotoxicology, Arch. Toxicol., pp. 659-675, (2015)

[10]

Cassano D., Bogni A., La Spina R., Gilliland D., Ponti J., Investigating the cellular uptake of model nanoplastics by single-cell ICP-MS, Nanomaterials, 594, (2023)

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