Assessment of salivary microbiota profile as a potential diagnostic tool for pediatric celiac disease

被引：0

作者：

Noruzpour, Asal ^{[1
]}

Gholam-Mostafaei, Fahimeh Sadat ^{[2
]}

Looha, Mehdi Azizmohammad ^{[3
]}

Dabiri, Hossein ^{[1
]}

Ahmadipour, Shokoufeh ^{[4
]}

Rouhani, Pejman ^{[5
]}

Ciacci, Carolina ^{[6
]}

Rostami-Nejad, Mohammad ^{[7
]}

机构：

[1] Shahid Beheshti Univ Medial Sci, Sch Med, Dept Microbiol, Tehran, Iran

[2] Shahid Beheshti Univ Med Sci, Res Inst Gastroenterol & Liver Dis, Gastroenterol & Liver Dis Res Ctr, Tehran, Iran

[3] Shahid Beheshti Univ Med Sci, Res Inst Gastroenterol & Liver Dis, Basic & Mol Epidemiol Gastrointestinal Disorders, Tehran, Iran

[4] Lorestan Univ Med Sci, Hepatitis Res Ctr, Khorramabad, Iran

[5] Shahid Beheshti Univ Med Sci, Mofid Childrens Hosp, Dept Pediat Gastroenterol & Hepatol, Tehran, Iran

[6] Univ Salerno, Dept Med & Surg, Salerno, Italy

[7] Shahid Beheshti Univ Med Sci, Res Inst Gastroenterol & Liver Dis, Celiac Dis & Gluten Related Disorders Res Ctr, Tehran, Iran

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

关键词：

Celiac disease; Salivary microbiota; Oral dysbiosis; Pediatric patients; GROUP-SPECIFIC PRIMERS; TIME PCR ANALYSIS; PREDOMINANT BACTERIA; GLUTEN; QUANTIFICATION; IDENTIFICATION; RISK;

D O I：

10.1038/s41598-024-67677-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The association between oral dysbiosis and celiac disease (CD) remains poorly understood, as does the impact of CD-associated dysbiosis on disease development or exacerbation. This study aims to investigate alterations in salivary microbial composition among children with CD. In this cross-sectional study, saliva samples from 12 children with active CD (A-CD group), 14 children with CD on a gluten-free diet (GFD), and 10 healthy control (HC) children were analyzed using DNA sequencing targeting the 16S ribosomal RNA. Both patients in A-CD and GFD groups showed a significant increase (p=0.0001) in the Bacteroidetes phylum, while the Actinobacteria phylum showed a significant decrease (p=0.0001). Notably, the Rothia genus and R.aeria also demonstrated a significant decrease (p=0.0001) within the both CD groups as compare to HC. Additionally, the control group displayed a significant increase (p=0.006) in R.mucilaginosa species compared to both CD patient groups. Distinct bacterial strains were abundant in the saliva of patients with active CD, indicating a unique composition of the salivary microbiome in individuals with CD. These findings suggest that our approach to assessing salivary microbiota changes may contribute to developing noninvasive methods for diagnosing and treating CD.

引用

页数：9

共 43 条

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