LncRNA MEG3 Inhibits the Epithelial-mesenchymal Transition of Bladder Cancer Cells through the Snail/E-cadherin Axis

被引:3
作者
Wang, Liang [1 ]
Wang, Ping [2 ]
Liu, Bing [3 ]
Zhang, Hui [1 ]
Wei, Cheng-cheng [1 ]
Xiong, Ming [1 ]
Luo, Gang [3 ]
Wang, Miao [1 ]
机构
[1] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Urol, Wuhan 430022, Peoples R China
[2] Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Clin Lab, Wuhan 430022, Peoples R China
[3] Huazhong Univ Sci & Technol, Cent Hosp Wuhan, Tongji Med Coll, Dept Urol, Wuhan 430014, Peoples R China
基金
中国国家自然科学基金;
关键词
long noncoding RNA; bladder cancer; maternally expressed gene 3; epithelial-mesenchymal transition; Snail; EMT; PROLIFERATION; EXPRESSION; INVASION; PATHWAY;
D O I
10.1007/s11596-024-2895-x
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
ObjectiveThis study aimed to investigate the role of the long noncoding RNA (lncRNA) maternally expressed gene 3 (MEG3) in the epithelial-mesenchymal transition (EMT) of bladder cancer cells and the potential mechanisms.MethodsCell invasion, migration, and wound healing assays were conducted to assess the effects of MEG3 on the invasive and migratory capabilities of bladder cancer cells. The expression levels of E-cadherin were measured using Western blotting, RT-qPCR, and dual luciferase reporter assays. RNA immunoprecipitation and pull-down assays were performed to investigate the interactions between MEG3 and its downstream targets.ResultsMEG3 suppressed the invasion and migration of bladder cancer cells and modulated the transcription of E-cadherin. The binding of MEG3 to the zinc finger region of the transcription factor Snail prevented its ability to transcriptionally repress E-cadherin. Additionally, MEG3 suppressed the phosphorylation of extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), and P38, thereby decreasing the expression of Snail and stimulating the expression of E-cadherin.ConclusionMEG3 plays a vital role in suppressing the EMT in bladder cancer cells, indicating its potential as a promising therapeutic target for the treatment of bladder cancer.
引用
收藏
页码:726 / 734
页数:9
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