Ultrasensitive electrochemical biosensors for the detection of mirna-21 based on au@3d-rgof-nh2 nanocomposite

An electrochemical sensor based on gold 3D amino functionalized reduction graphene oxide （Au@3DrGOF-NH2） nanocomposite was designed to detect miRNA-21. The surface morphology， elemental composition and surface groups of the materials were characterized by scanning electron microscopy， transmission electron microscopy and X-ray diffraction. The response signal of electrochemical sensor was measured by differential pulse voltammetry. Under the optimal experimental conditions， the standard curve for the detection of miRNA-21 was ΔI（μA）=13.159lgc+185.87， and the correlation coefficient R2 was 0.9967， showing a good response in the linear range of 1.0×10-13-1.0×10-6 mol/L. The limit of detection （LOD） was 4.34×10-14 mol/L. The spiked recoveries of serum samples were 98.4%-101.2%. The electrochemical sensor can be used for the determination of miRNA-21 in serum. © 2023, Youke Publishing Co.,Ltd. All rights reserved.