Mitochondrial DNA and Inflammation Are Associated with Cerebral Vessel Remodeling and Early Diabetic Kidney Disease in Patients with Type 2 Diabetes Mellitus

被引：4

作者：

Petrica, Ligia ^{[1
,2
,3
,4
,5
]}

Gadalean, Florica ^{[1
,2
,5
]}

Muntean, Danina Mirela ^{[2
,4
,6
]}

Jianu, Dragos Catalin ^{[2
,3
,5
,7
]}

Vlad, Daliborca ^{[2
,5
,8
]}

Dumitrascu, Victor ^{[2
,5
,8
]}

Bob, Flaviu ^{[1
,2
,5
]}

Milas, Oana ^{[1
,2
,5
]}

Suteanu-Simulescu, Anca ^{[1
,2
,5
]}

Glavan, Mihaela ^{[1
,2
,5
]}

Ursoniu, Sorin ^{[2
,3
,4
,5
,9
]}

Balint, Lavinia ^{[1
,2
,5
]}

Mogos-Stefan, Maria ^{[1
,2
,5
]}

Ienciu, Silvia ^{[1
,2
,5
]}

Cretu, Octavian Marius ^{[10
,11
]}

Popescu, Roxana ^{[2
,5
,12
]}

Gluhovschi, Cristina ^{[1
,2
]}

Iancu, Lavinia ^{[1
,2
,5
]}

Vlad, Adrian ^{[2
,5
,13
]}

机构：

[1] Victor Babes Univ Med & Pharm, Dept Internal Med 2, Div Nephrol, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[2] Victor Babes Univ Med & Pharm, Fac Med, Ctr Mol Res Nephrol & Vasc Dis, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[3] Victor Babes Univ Med & Pharm, Fac Med, Ctr Cognit Res Neuropsychiat Pathol Neuropsy Cog, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[4] Victor Babes Univ Med & Pharm, Fac Med, Ctr Translat Res & Syst Med, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[5] Cty Emergency Hosp Timisoara, Timisoara 300723, Romania

[6] Victor Babes Univ Med & Pharm, Dept Funct Sci 3, Div Pathophysiol, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[7] Victor Babes Univ Med & Pharm, Dept Neurosciences8, Div Neurology1, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[8] Victor Babes Univ Med & Pharm, Dept Biochem & Pharmacology4, Div Pharmacol, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[9] Victor Babes Univ Med & Pharm, Dept Funct Sci 3, Div Publ Hlth & Hist Med, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[10] Victor Babes Univ Med & Pharm, Dept Surg 1, Div Surg Semiol 1, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[11] Emergency Clin Municipal Hosp Timisoara, Timisoara 300041, Romania

[12] Victor Babes Univ Med & Pharm, Dept Microscop Morphol 2, Div Cell & Mol Biol 2, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

[13] Victor Babes Univ Med & Pharm, Dept Internal Med 2, Div Diabet, 2,Eftimie Murgu Sq, Timisoara 300041, Romania

来源：

BIOMOLECULES | 2024年 / 14卷 / 04期

关键词：

mitochondrial DNA; inflammation; cerebral vessels; diabetic kidney disease; CIRCULATING ADHESION MOLECULES; INTIMA-MEDIA THICKNESS; INTERNAL CAROTID-ARTERY; CORONARY-HEART-DISEASE; CEREBROVASCULAR REACTIVITY; COPY NUMBER; TNF-ALPHA; ATHEROSCLEROSIS; RISK; INTERLEUKIN-18;

D O I：

10.3390/biom14040499

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cerebrovascular disease accounts for major neurologic disabilities in patients with type 2 diabetes mellitus (DM). A potential association of mitochondrial DNA (mtDNA) and inflammation with cerebral vessel remodeling in patients with type 2 DM was evaluated. A cohort of 150 patients and 30 healthy controls were assessed concerning urinary albumin/creatinine ratio (UACR), synaptopodin, podocalyxin, kidney injury molecule-1 (KIM-1), N-acetyl-beta-(D)-glucosaminidase (NAG), interleukins IL-17A, IL-18, IL-10, tumor necrosis factor-alpha (TNF alpha), intercellular adhesion molecule-1 (ICAM-1). MtDNA-CN and nuclear DNA (nDNA) were quantified in peripheral blood and urine by qRT-PCR. Cytochrome b (CYTB) gene, subunit 2 of NADH dehydrogenase (ND2), and beta 2 microglobulin nuclear gene (B2M) were assessed by TaqMan assays. mtDNA-CN was defined as the ratio of the number of mtDNA/nDNA copies, through analysis of the CYTB/B2M and ND2/B2M ratio; cerebral Doppler ultrasound: intima-media thickness (IMT)-the common carotid arteries (CCAs), the pulsatility index (PI) and resistivity index (RI)- the internal carotid arteries (ICAs) and middle cerebral arteries (MCAs), the breath-holding index (BHI). The results showed direct correlations of CCAs-IMT, PI-ICAs, PI-MCAs, RI-ICAs, RI-MCAs with urinary mtDNA, IL-17A, IL-18, TNF alpha, ICAM-1, UACR, synaptopodin, podocalyxin, KIM-1, NAG, and indirect correlations with serum mtDNA, IL-10. BHI correlated directly with serum IL-10, and serum mtDNA, and negatively with serum IL-17A, serum ICAM-1, and NAG. In neurologically asymptomatic patients with type 2 DM cerebrovascular remodeling and impaired cerebrovascular reactivity may be associated with mtDNA variations and inflammation from the early stages of diabetic kidney disease.

引用

页数：21

共 63 条

[1] Diabetes, the metabolic syndrome, and ischemic stroke [J].