Aerobic denitrifying bacteria community structure and response of aerobic denitrifying bacteria to dissloved organic matter in the sediments in Lake Baiyangdian in the spring

被引：0

作者：

Chen Z. ^{[1
]}

Zhang T. ^{[1
]}

Zhang Z. ^{[1
]}

Zhou S. ^{[1
]}

Dong W. ^{[1
]}

Cui J. ^{[1
]}

Luo X. ^{[1
]}

机构：

[1] Pollution Prevention Biotechnology Laboratory of Hebei Province, School of Environmental Science and Enginnering, Hebei University of Science and Technology, Shijiazhuang

来源：

Hupo Kexue/Journal of Lake Sciences | 2022年 / 34卷 / 02期

关键词：

Aerobic denitrification; Dissolved organic matter; Fluorescence region integral method; Lake Baiyangdian; Responding mechanism; Sediment;

D O I：

10.18307/2022.0215

中图分类号：

学科分类号：

摘要：

Aerobic denitrification has become a research hotspot in biological denitrification in recent years due to its unique advantages. Dissolved organic matter (DOM) as a carbon source is the important reason for community differences. To explore the responding mechanism of aerobic denitrifying bacteria community structure to dissolved organic matter in different functional areas of Lake Baiyangdian, this paper combines the fluorescence area integration method and the high-throughput sequencing technology of napA denitrification genes to study the characteristics of the aerobic denitrifying bacterial community structure and responding mechanism in Lake Baiyangdian. The results showed that the concentration of protein-like components was higher than that of humic-like components in the organic matter of Lake Baiyangdian sediments in spring. Among them, the protein-like components in the culture area were the largest, reaching 79.63%±3.79%, and the humus-like component in the primitive area was the largest, reaching 33.91%±6.32%; A total of 3693 OTUs were acquired high Throughput sequencing, which was divided into 9 main phyla. Among them, the Proteobacteria accounted for the largest proportion, reaching over 99%; the α diversity Chao1 index showed significant spatial differences, and the travel area>the living area>the entry area>the original area>the breeding area; the main species of aerobic denitrifying bacteria include Aeromonas, Sulfuritortus, Cupriavidus, Pseudomonas and Thaurea, Ferrimonas, as an indicator species, contributes the most to the difference; Redundant analysis found that the components of fulvic acid-like substances and microbial metabolites in the visible light area are the main reasons for the differences in aerobic denitrifying bacteria in different functional areas. In summary, this research will not only help to further understand the characteristics of the nitrogen cycle microorganisms in the natural environment; it will also provide a reference for the selection of carbon sources suitable for efficient bacterial screening in the practical environment. © 2022 by Journal of Lake Sciences.

引用

页码：538 / 552

页数：14

共 48 条

[1] Cui QQ, Pan YT, Zhang HX, Et al., Elevated concentrations of perfluorohexanesulfonate and other per-and polyfluoroalkyl substances in Lake Baiyangdian (China): Source characterization and exposure assessment, Environmental Pollution, 241, pp. 684-691, (2018)
[2] Zhou SL, Sun Y, Yue GC, Et al., Vertical distribution characteristics and driving factors of microbial community of spring culture area sediments in Lake Baiyangdian, Acta Scientiae Circumstantiae, 40, 5, pp. 1722-1733, (2020)
[3] Ji B, Yang K, Zhu L, Et al., Aerobic denitrification: A review of important advances of the last 30 years, Biotechnology and Bioprocess Engineering, 20, 4, pp. 643-651, (2015)
[4] Mu DY, Jin PF, Peng YZ, Et al., Identification and nitrogen removal characteristics of the heterotrophic nitrification and aerobic denitrification bacterial strain DK1, Environmental Science, 38, 11, pp. 4763-4773, (2017)
[5] Zhou SL, Huang TL, Bai SY, Et al., Isolation, Identification, and nitrogen removal characteristics of oligotrophic aerobic denitrifiers, China Environmental Science, 36, 1, pp. 238-248, (2016)
[6] Jia YT, Zhou MM, Chen YC, Et al., Carbon selection for nitrogen degradation pathway by Stenotrophomonas maltophilia: Based on the balances of nitrogen, carbon and electron, Bioresource Technology, 294, (2019)
[7] Bai J, Chen L, Huang X, Et al., Isolation and nitrogen removal characteristics of salt-tolerant heterotrophic nitrification and aerobic denitrification bacteria Zobellella sp. B307, Environmental Science, 39, 10, pp. 4793-4801, (2018)
[8] Wang HY, Ma F, Su JF, Et al., Influence of carbon source and C/N ratio on nitrogen removal of aerobic denitrifier, Acta Scientiae Circumstantiae, 27, 6, pp. 968-972, (2007)
[9] Wen G, Wang T, Li K, Et al., Aerobic denitrification performance of strain Acinetobacter johnsonii WGX-9 using different natural organic matter as carbon source: Effect of molecular weight, Water Research, 164, (2019)
[10] Zhou SL, Zhang TN, Chen ZY, Et al., Response of aerobic denitrification bacterial community structure to dissolved organic matter in Lake Baiyangdian during the water delivery period, Environmental Science, (2021)

← 1 2 3 4 5 →