Uncovering the genetic diversity in Aedes aegypti insecticide resistance genes through global comparative genomics

被引：4

作者：

Spadar, Anton ^{[1
]}

Collins, Emma ^{[1
]}

Messenger, Louisa A. ^{[2
,3
]}

Clark, Taane G. ^{[1
,4
]}

Campino, Susana ^{[1
]}

机构：

[1] London Sch Hyg & Trop Med, Fac Infect & Trop Dis, Dept Infect Biol, London, England

[2] Univ Nevada, Sch Publ Hlth, Dept Environm & Occupat Hlth, Las Vegas, NV USA

[3] Univ Nevada, Sch Publ Hlth, Parasitol & Vector Biol Lab, UNLV PARAVEC Lab, Las Vegas, NV USA

[4] London Sch Hyg & Trop Med, Fac Epidemiol & Populat Hlth, London, England

来源：

SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;

关键词：

Aedes aegypti; Insecticide resistance; Vector-borne disease; Genomics; MOSQUITO PREFERENCE; VECTOR; TRANSFERASE; EVOLUTION; CHANNELS; CLIMATE; DENGUE;

D O I：

10.1038/s41598-024-64007-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aedes aegypti is vector of many arboviruses including Zika, dengue, yellow fever, West Nile, and Chikungunya. Its control efforts are hampered by widespread insecticide resistance reported in the Americas and Asia, while data from Africa is more limited. Here we use publicly available 729 Ae. aegypti whole-genome sequencing samples from 15 countries, including nine in Africa, to investigate the genetic diversity in four insecticide resistance linked genes: ace-1, GSTe2, rdl and vgsc. Apart from vgsc, the other genes have been less investigated in Ae. aegypti, and almost no genetic diversity information is available. Among the four genes, we identified 1,829 genetic variants including 474 non-synonymous substitutions, some of which have been previously documented, as well as putative copy number variations in GSTe2 and vgsc. Global insecticide resistance phenotypic data demonstrated variable resistance in geographic areas with resistant genotypes. Overall, our work provides the first global catalogue and geographic distribution of known and new amino-acid mutations and duplications that can be used to guide the identification of resistance drivers in Ae. aegypti and thereby support monitoring efforts and strategies for vector control.

引用

页数：12

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