Determination of Clotrimazole Residue in Animal-Derived Food by Gas Chromatography-Tandem Mass Spectrometry with Isotope Dilution

被引：0

作者：

Han C. ^{[1
]}

Hu B. ^{[2
]}

Li Z. ^{[3
]}

Zhu Z. ^{[3
]}

Shen Y. ^{[1
]}

Zhou X. ^{[4
]}

机构：

[1] College of Biological and Environmental Engineering, Zhejiang Shuren University, Hangzhou

[2] Shaoxing Customs Comprehensive Technical Service Center, Shaoxing

[3] Wenzhou Customs Comprehensive Technical Service Center, Wenzhou

[4] Zhoushan Customs Comprehensive Technical Service Center, Zhoushan

来源：

Science and Technology of Food Industry | 2021年 / 42卷 / 05期

关键词：

animal-derived food; clotrimazole; gas chromatography-tandem mass spectrometry; isotope dilution; residue;

D O I：

10.13386/j.issn1002-0306.2020030010

中图分类号：

学科分类号：

摘要：

An isotope dilution gas chromatography-tandem mass spectrometry(GC-MS/MS)method for the determination of clotrimazole residues in animal-derived food(Pork,chicken,pig fat,pig heart,pig liver,pig kidney)was established. The samples were extracted with accelerated solvent extraction,and cleaned up by gel permeation chromatography. HP-5 MS chromatographic column(30 m×0.25 mm×0.25 μm)was used for separation,multi-reaction monitoring(MRM)mode detection,and isotope internal standard method for quantification. Results showed that there was a good linearity for clotrimazole in the range of 1~100 μg/L with a correlation coefficient(R2)of 0.9995. The limit of quantitation(LOQ,S/N≥10)of the method was 2.0 μg/kg. The average recoveries at three spiking levels(2.0,5.0,20.0 μg/kg)were 82.6%~116.5%. Relative standard deviation(RSD)were 5.2%~13.5%. This method has high sensitivity and accuracy,and can be used for the confirmation and quantitative analysis of clotrimazole residues in animal-originated foodstuffs. © 2021 Science and Technology of Food Industry. All rights reserved.

引用

页码：216 / 220

页数：4

共 9 条

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