Enzymatically produced pectic-oligosaccharides from fruit waste of Citrus reticulata (mandarin) peels display cytotoxicity against colon cancer cells

被引：4

作者：

Rajulapati V. ^{[1
]}

Dhillon A. ^{[1
]}

Goyal A. ^{[1
]}

机构：

[1] Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam

来源：

Bioresource Technology Reports | 2021年 / 15卷

关键词：

Biocompatible; Cytotoxicity; Mandarin peel waste; Pectic oligosaccharides; Pectin; Ultrasound assisted extraction;

D O I：

10.1016/j.biteb.2021.100740

中图分类号：

学科分类号：

摘要：

Pectin was extracted from Citrus reticulata (mandarin) peels by ultrasound assisted extraction process. The yield of extracted mandarin pectin (EMP) was 21.3 ± 1.2%. EMP after purification (pEMP) by size-exclusion chromatography gave molecular mass, 92.3 kDa. Field-emission scanning electron microscopy image of pEMP showed wrinkled surface and Atomic force microscopy revealed fibrous network. Fourier transform infrared spectroscopic and nuclear magnetic resonance analyses of pEMP showed hetero-polysaccharide nature with 68 ± 2% methylated galacturonic acids. Thermogravimetric analysis showed degradation at 225 ± 5 °C. pEMP treated by recombinant clostridial pectate-lyase (CtPL1B) or a mixture of CtPL1B and pectin-methylesterase (CtPME) produced pectic-oligosaccharides having degree of polymerization, DP2 and DP3. Enzyme assays displayed that CtPME hydrolyzed methyl groups from pEMP and resulting pectate was degraded by CtPL1B. ESI-MS analysis confirmed that the treatment of pEMP by CtPL1B produces methylated unsaturated di-galacturonic and tri-galacturonic acids, which, displayed biocompatibility with normal human kidney (HEK293) cells and cytotoxicity against colon cancer (HT29) cells. © 2021 Elsevier Ltd

引用

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