Complete genome sequence of Bacillus velezensis strain Ag109, a biocontrol agent against plant-parasitic nematodes and Sclerotinia sclerotiorum

被引：2

作者：

Mian, Silas ^{[1
]}

Machado, Andressa Cristina Zamboni ^{[2
]}

Hoshino, Rodrigo Thibes ^{[1
]}

Mosela, Mirela ^{[3
]}

Higashi, Allan Yukio ^{[1
]}

Shimizu, Gabriel Danilo ^{[1
]}

Teixeira, Gustavo Manoel ^{[3
]}

Nogueira, Alison Fernando ^{[1
]}

Giacomin, Renata Mussoi ^{[4
]}

Ribeiro, Luriam Aparecida Brandao ^{[2
]}

Koltun, Alessandra ^{[5
]}

de Assis, Rafael ^{[1
]}

Goncalves, Leandro Simoes Azeredo ^{[1
]}

机构：

[1] Univ Estadual Londrina, Agron Dept, BR-86097570 Londrina, PR, Brazil

[2] Inst Agron Parana, Nematol Dept, BR-86047902 Londrina, PR, Brazil

[3] Univ Estadual Londrina, Microbiol Dept, BR-86097570 Londrina, PR, Brazil

[4] Univ Estadual Centro Oeste, Biol Dept, BR-85015430 Guarapuava, PR, Brazil

[5] Univ Estadual Campinas, Ctr Mol Biol & Genet Engn, BR-13083875 Campinas, SP, Brazil

来源：

BMC MICROBIOLOGY | 2024年 / 24卷 / 01期

关键词：

Glycine max (L.) Merr; Biological control; Sclerotinia sclerotiorum; Meloidogyne [!text type='java']java[!/text]nica; Pratylenchus brachyurus; LIPOPEPTIDES; MANAGEMENT; REVEALS; BIOLOGY;

D O I：

10.1186/s12866-024-03282-9

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Soybean is the main oilseed cultivated worldwide. Even though Brazil is the world's largest producer and exporter of soybean, its production is severely limited by biotic factors. Soil borne diseases are the most damaging biotic stressors since they significantly reduce yield and are challenging to manage. In this context, the present study aimed to evaluate the potential of a bacterial strain (Ag109) as a biocontrol agent for different soil pathogens (nematodes and fungi) of soybean. In addition, the genome of Ag109 was wholly sequenced and genes related to secondary metabolite production and plant growth promotion were mined. Ag109 showed nematode control in soybean and controlled 69 and 45% of the populations of Meloidogyne javanica and Pratylenchus brachyurus, respectively. Regarding antifungal activity, these strains showed activity against Macrophomia phaseolina, Rhizoctonia solani, and Sclerotinia sclerotiorum. For S. sclerotiorum, this strain increased the number of healthy plants and root dry mass compared to the control (with inoculation). Based on the average nucleotide identity and digital DNA-DNA hybridization, this strain was identified as Bacillus velezensis. Diverse clusters of specific genes related to secondary metabolite biosynthesis and root growth promotion were identified, highlighting the potential of this strain to be used as a multifunctional microbial inoculant that acts as a biological control agent while promoting plant growth in soybean.

引用

页数：13

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