Characteristics of Antibiotic Resistance Genes in Various Livestock Feedlot Soils of the Hilly Purple Soil Region

被引：3
作者：
Cheng J.-H. [1 ,2 ]
Tang X.-Y. [1 ]
Liu C. [1 ]
机构：
[1] Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu
[2] University of Chinese Academy of Sciences, Beijing
来源：
Huanjing Kexue/Environmental Science | 2019年 / 40卷 / 07期
关键词：
Antibiotic resistance genes (ARGs); High-throughput QPCR (HT-QPCR); Livestock feedlot; Mobile genetic elements (MGEs); Purple soil;
D O I：
10.13227/j.hjkx.201811086
中图分类号：
学科分类号：
摘要：
Given the potential risk posed by antibiotic resistance genes (ARGs) to the eco-environment in the hilly purple soil region, which has been intensively utilized by human, surface soil samples were collected from feedlots of pig, chicken, and cattle farms and were analyzed for the diversity and abundance of ARGs using high-throughput QPCR. In total, 79 ARGs and 5 mobile genetic elements (MGEs) were detected across all samples. Among these genes, multidrug resistance genes were the most abundant type of ARGs. More abundant and diverse ARGs were observed in feedlot soil samples from pig and chicken farms than those from cattle feedlot soils, and these samples showed different distribution patterns of ARGs. High abundance of MGEs and their significant correlation with ARGs (P<0.05) implied an important role of MGEs in the dissemination of ARGs in livestock feedlot soils. © 2019, Science Press. All right reserved.
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页码：3257 / 3262
页数：5
相关论文
共 29 条
  • [1] Pruden A., Pei R.T., Storteboom H., Et al., Antibiotic resistance genes as emerging contaminants: studies in northern Colorado, Environmental Science & Technology, 40, 23, pp. 7445-7450, (2006)
  • [2] Zhu Y.G., Ouyang W.Y., Wu N., Et al., Antibiotic resistance: sources and mitigation, Bulletin of Chinese Academy of Sciences, 30, 4, pp. 509-516, (2015)
  • [3] Su J.Q., Huang F.Y., Zhu Y.G., Antibiotic resistance genes in the environment, Biodiversity Science, 21, 4, pp. 481-487, (2013)
  • [4] D'Costa V.M., King C.E., Kalan L., Et al., Antibiotic resistance is ancient, Nature, 477, 7365, pp. 457-461, (2011)
  • [5] Knapp C.W., Dolfing J., Ehlert P.A.I., Et al., Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940, Environmental Science & Technology, 44, 2, pp. 580-587, (2010)
  • [6] Looft T., Johnson T.A., Allen H.K., Et al., In-feed antibiotic effects on the swine intestinal microbiome, Proceedings of the National Academy of Sciences of the United States of America, 109, 5, pp. 1691-1696, (2012)
  • [7] Chee-Sanford J.C., Mackie R.I., Koike S., Et al., Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste, Journal of Environmental Quality, 38, 3, pp. 1086-1108, (2009)
  • [8] Zhu Y.G., Johnson T.A., Su J.Q., Et al., Diverse and abundant antibiotic resistance genes in Chinese swine farms, Proceedings of the National Academy of Sciences of the United States of America, 110, 9, pp. 3435-3440, (2013)
  • [9] He L.Y., Liu Y.S., Su H.C., Et al., Dissemination of antibiotic resistance genes in representative broiler feedlots environments: identification of indicator ARGs and correlations with environmental variables, Environmental Science & Technology, 48, 22, pp. 13120-13129, (2014)
  • [10] Forsberg K.J., Reyes A., Wang B., Et al., The shared antibiotic resistome of soil bacteria and human pathogens, Science, 337, 6098, pp. 1107-1111, (2012)
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