Genomic distinctiveness and recombination in tomato leaf curl New Delhi virus (ToLCNDV-BG) isolates infecting bitter gourd

There are two begomoviruses, tomato leaf curl New Delhi virus (ToLCNDV) and bitter gourd yellow mosaic virus (BgYMV) infecting bitter gourd in India. An extensive survey conducted from 2019 to 2022 clearly established that infection by ToLCNDV is more predominant (92.43%) than BgYMV (44%). The ToLCNDV isolates infecting bitter gourd shared only 88% identity in the DNA-A component with other ToLCNDV isolates and were found to be a distinct variant. The predicted amino acid sequence of the viral proteins, replication initiation protein, coat protein, and the symptom determinant protein in the study isolates are markedly different. Especially the RCR motif I and RCR motif III are different from other geminiviruses. Infectivity of cloned components of one of the isolates ToLCNDV-BG NP was demonstrated in bitter gourd. Recombination analysis clearly revealed that the study isolates are recombinants with the major parent predicted as squash leaf curl Yunnan virus (GenBank Accession Number: MK064241) and the minor parent as ToLCNDV from Pakistan (GenBank Accession Number: AM747291). Due to distinct genomic features and less than 90% identity with the majority of ToLCNDV isolates, the study isolates deserve to be raised to the status of a distinct strain, designated as ToLCNDV-BG.