Anti-obesity Effect of Pteridium aquilinum Extract and Its Mechanism

被引：0
|
作者：
Wu Y. [1 ,2 ]
Kim Y. [2 ]
Kwon T. [2 ]
Bi S. [1 ]
Kim T. [2 ]
机构：
[1] College of Life and Environment Science, Huangshan University, Huangshan, 245041, Anhui
[2] Department of Food Science and Biotechnology, Andong National University, Andong
来源：
Journal of Chinese Institute of Food Science and Technology | 2019年 / 19卷 / 10期
关键词：
3T3-L1; adipocytes; Anti-obesity; C57BL/6J obese mice; Molecular mechanism; Pteridium aquilinum;
D O I：
10.16429/j.1009-7848.2019.10.005
中图分类号：
学科分类号：
摘要：
Anti-obesity effect of Pteridium aquilinum and its mechanism was investigated by measuring its inhibitory effect against 3T3-L1 preadipocyte differentiation and high fat diet induced C57BL/6J obese mice. The differentiation of 3T3-L1 cells was induced and Oil Red O staining was used to analyze the effect of Pteridium aquilinum ethanol extract (PAE) on the differentiation of 3T3-L1 preadipocytes. To elucidate the mechanism of PAE, we performed Western blot for the expression of genes related with adipogenesis (PPARγ, CEBPα, SREBP-1c) and lipogenesis (FAS, ACC). C57BL/6J mice were induced by high fat diet for 6 weeks. Obese C57BL/6J mice were then randomly divided into high-fat diet group (HFD) and the 1%PAE group (HFD+PAE). In addition, C57BL/6J mice fed with normal diet were selected as the normal diet group (ND). After 8 weeks, the body weight, glucose metabolism indications (blood glucose, glucose tolerance) and serum lipid levels (TG, TC) in all mice were evaluated. The results showed that PAE suppressed 3T3-L1 adipocyte differentiation and reduced the accumulation of fat in adipocytes. Treatment of PAE markedly suppressed the protein expression of PPARγ, CEBPα and SREBP-1c, as well as FAS and ACC (P<0.05), which are the key transcription factors and metabolic enzymes in adipogenesis and lipogenesis. After 8-weeks feeding, the indicator in HFD group were high than ND group, including body weight, body fat deposition, serum glucose and serum lipid (P<0.05). Treatment with PAE significantly decreased the body weight, blood glucose and the levels of serum TC, TG. PAE also effectively improved impaired glucose tolerance (P<0.05). In conclusion, Pteridium aquilinum suppressed 3T3-L1 adipocyte differentiation, inhibited obesity, hyperglycemia, hyperlipemia and improved impaired glucose tolerance in C57BL/6J obese mice. These effects might be closed related to its inhibition of genes expression regulating adipogenesis and lipogenesis. © 2019, Editorial Office of Journal of CIFST. All right reserved.
引用
收藏
页码：40 / 48
页数：8
相关论文
共 14 条
  • [1] Kuo D.H., Yeh C.H., Shieh P.C., Et al., Effect of shanzha, a Chinese herbal product, on obesity and dyslipidemia in hamsters receiving high-fat diet, Journal of Ethnopharmacology, 124, 3, pp. 544-550, (2009)
  • [2] Tsumbu C.N., Deby-Dupont G., Tits M., Et al., Polyphenol content and modulatory activities of some tropical dietary plant extracts on the oxidant activities of neutrophils and myeloperoxidase, International Journal of Molecular Sciences, 13, 1, pp. 628-650, (2012)
  • [3] Mohammad R.H., Nur-E-Alam M., Lahmann M., Et al., Isolation and characterisation of 13 pterosins and pterosides from bracken (Pteridium aquilinum(L.) Kuhn) rhizome, Phytochemistry, 128, pp. 82-94, (2016)
  • [4] Chen N.D., Chen N.F., Chen C.W., Et al., Separation and structure elucidation of a new homoflavanol derivative from Pteridium aquilinum(L.) Kuhn, Natural Product Research, 27, 19, pp. 1764-1769, (2013)
  • [5] Xu W., Zhang F., Luo Y., Et al., Antioxidant activity of a water-soluble polysaccharide purified from Pteridium aquilinum, Carbohydrate Research, 344, 2, pp. 217-222, (2009)
  • [6] Song G., Wang K., Zhang H., Et al., Structural characterization and immunomodulatory activity of a novel polysaccharide from Pteridium aquilinum, International Journal of Biological Macromolecules, 102, pp. 599-604, (2017)
  • [7] Wang H., Wu S., Preparation and antioxidant activity of Pteridium aquilinum-derived oligosaccharide, International Journal of Biological Macromolecules, 61, 10, pp. 33-35, (2013)
  • [8] Wu Y.X., Kim Y.J., Li S., Et al., Anti-obese effects of mulberry (Morus alba L.) Root Bark through the Inhibition of Digestive Enzymes and 3T3-L1 Adipocyte differentiation, Korean Journal of Food Preservation, 22, 1, pp. 27-35, (2015)
  • [9] Spiegelman B.M., Flier J.S., Adipogenesis and obesity: rounding out the big picture, Cell, 87, 3, pp. 377-389, (1996)
  • [10] White U.A., Stephens J.M., Transcriptional factors that promote formation of white adipose tissue, Molecular and Cellular Endocrinology, 318, 1-2, pp. 10-14, (2010)
12