New Zealand Journal of Forestry Science Effect of light intensity and seal type on the in vitro elongation and adventitious rooting of Eucalyptus grandis × E. urophylla

被引：0
作者：
Souza D.M.S.C. [1 ]
Fernandes S.B. [1 ]
Duarte V.P. [2 ]
Molinari L.V. [1 ]
Teixeira G.L. [3 ]
Brondani G.E. [1 ]
机构：
[1] Laboratory of in vitro Culture of Forest Species, Department of Forestry Sciences, Federal University of Lavras, Minas Gerais, Lavras
[2] Institute of Natural Resources, Federal University of Itajubá, University Campus, MG, Itajubá
[3] Institute of Agricultural Science, Federal University of Minas Gerais, University Campus, Minas Gerais, Montes Claros
来源：
New Zealand Journal of Forestry Science | 2024年 / 54卷
关键词：
clonal propagation; luminosity; LED; micropropagation; porous membranes;
D O I：
10.33494/nzjfs542024x284x
中图分类号：
学科分类号：
摘要：
Background: Rejuvenation/reinvigoration of tissues through micropropagation has become an important tool for clonal propagation in eucalypts species. This study evaluated the effect of photomixotrophism (i.e., light intensity and seal type) on in vitro elongation and adventitious rooting to identify the limiting factors on in vitro culture of the Eucalyptus grandis × E. urophylla hybrid. Methods: Nodal segments (i.e., explants) from ministumps grown in a semi-hydroponic system were collected. The effects of light intensity and seal type on in vitro elongation and adventitious rooting stages were evaluated from a 40 µmol m-2 s-1 fluorescent lamp and 20, 40, and 80 µmol m-2 s-1 red/blue LEDs, with (through porous membranes) and without gas exchange. Results: Based on the results at 35 d, 40 µmol m-2 s-1 fluorescent lamp and gas exchange combination was the most suitable for in vitro elongation and adventitious rooting of Eucalyptus grandis × E. urophylla. Both factors increased the vigour, shoot length, photosynthetic pigment content, xylem, phloem, stomatal number and density, root length, diameter, number of roots per explant, and adventitious rooting fraction. Conclusions: Light intensity and seal type influences the in vitro elongation and adventitious rooting of Eucalyptus grandis × E. urophylla. The results contribute to optimising the cloning of commercial eucalypts species by the micropropagation technique. © The Author(s).
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共 55 条
[1]  
Abiri R., Atabaki N., Abdul-Hamid H., Sanusi R., Ab Shukor N.A., Shaharuddin N.A., Malik S., The prospect of physiological events associated with the micropropagation of Eucalyptus sp, Forests, 11, 11, (2020)
[2]  
Almeida M.R., Aumond M., Costa C.T., Schwambach J., Ruedell C.M., Correa L.R., Fett-Neto A.G., Environmental control of adventitious rooting in Eucalyptus and Populus cuttings, Trees, 31, pp. 1377-1390, (2017)
[3]  
Baccarin F.J.B., Brondani G.E., Almeida L.V., Vieira I.G., Oliveira L.S., Almeida M., Vegetative rescue and cloning of Eucalyptus benthamii selected adult trees, New Forests, 46, pp. 465-483, (2015)
[4]  
Batista D.S., Castro K.M., Silva A.R., Teixeira M.L., Sales T.A., Soares L.I., Otoni W.C., Light quality affects in vitro growth and essential oil profile in Lippia alba (Verbenaceae), In Vitro Cellular & Developmental Biology-Plant, 52, pp. 276-282, (2016)
[5]  
Batista D.S., Felipe S.H.S., Silva T.D., Castro K.M., Mamedes-Rodrigues T.C., Miranda N.A., Rios-Rios A.M., Faria D.V., Fortini E.A., Chagas K., Torres-Silva G., Xavier A., Arencibia A.D., Otoni W.C., Light quality in plant tissue culture: does it matter?, In Vitro Cellular & Developmental Biology-Plant, 54, pp. 195-215, (2018)
[6]  
Brondani G.E., Oliveira L.S., Konzen E.R., Silva A.L.L., Costa J.C., Mini-incubators improve the adventitious rooting performance of Corymbia and Eucalyptus microcuttings according to the environment in which they are conditioned, Anais da Academia Brasileira de Ciências, 90, 2, pp. 2409-2423, (2018)
[7]  
Carrillo I., Mendonca R.T., Ago M., Rojas O.J., Comparative study of cellulosic components isolated from different Eucalyptus species, Cellulose, 25, pp. 1011-1029, (2018)
[8]  
Castro E.M., Pinto J.E.B.P., Soares A.M., Melo H.C., Bertalucci S.K.V., Vieira C.V., Junior E.C.L., Anatomic adaptation of Mikania glomerata Sprengel (Asteraceae) leaves in three distinct regions of the plant under different shading levels, Revista Brasileira de Plantas Medicinais, 9, 2, pp. 8-16, (2007)
[9]  
Coelho A.D., Souza C.K., Bertolucci S.K.V., Carvalho A.A., Santos G.C., Oliveira T., Marques E.A., Salimena J.P., Pinto J.E.B.P., Wavelength and light intensity enhance growth, phytochemical contents and antioxidant activity in micropropagated plantlets of Urtica dioica L, Plant Cell, Tissue and Organ Culture, 145, pp. 59-74, (2021)
[10]  
Diaz-Sala C., A perspective on adventitious root formation in tree species, Plants, 9, 12, (2020)
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