Clinical Evaluation of the BIOFIRE SPOTFIRE Respiratory Panel

被引：3

作者：

Chan, Wai-Sing ^{[1
]}

Ho, Christy Wing-Yiu ^{[1
]}

Chan, Tsz-Ching ^{[1
]}

Hung, Jeffrey ^{[1
]}

To, Man-Yan ^{[1
]}

Leung, Sau-Man ^{[1
]}

Lai, Ka-Chun ^{[1
]}

Wong, Ching-Yan ^{[1
]}

Leung, Chin-Pang ^{[1
]}

Au, Chun-Hang ^{[1
]}

Wan, Thomas Shek-Kong ^{[1
]}

Zee, Jonpaul Sze-Tsing ^{[1
]}

Ma, Edmond Shiu-Kwan ^{[1
]}

Tang, Bone Siu-Fai ^{[1
]}

机构：

[1] Hong Kong Sanat & Hosp, Dept Pathol, Hong Kong, Peoples R China

来源：

VIRUSES-BASEL | 2024年 / 16卷 / 04期

关键词：

BIOFIRE; FilmArray; flu; PCR; respiratory; SARS-CoV-2; SPOTFIRE; TORCH;

D O I：

10.3390/v16040600

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The BIOFIRE SPOTFIRE Respiratory (R) Panel is a novel, in vitro diagnostic PCR assay with 15 pathogen targets. The runtime is about 15 min which is the shortest among similar panels in the market. We evaluated the performance of the SPOTFIRE R Panel with 151 specimens, including 133 collected from the upper respiratory tract (URT), 13 from the lower respiratory tract (LRT) and 5 external quality assessment program (EQAP) samples. The respiratory specimens were enrolled throughout the first two post-COVID-19 influenza seasons in Hong Kong (March to December 2023). For URT specimens, full concordance was observed between the SPOTFIRE R Panel and the standard-of-care FilmArray Respiratory 2.1 plus Panel (RP2.1plus) for 109 specimens (109/133, 81.95%). After discrepant analysis, the SPOTFIRE R Panel identified more pathogens than the RP2.1plus in 15 specimens and vice versa in 3 specimens. The per-target negative and positive percentage agreement (NPA and PPA) were 92.86-100% except the PPA of adenovirus (88.24%). For LRT and EQAP samples, all results were fully concordant. To conclude, the performance of the SPOTFIRE R Panel was comparable to the RP2.1plus.

引用

页数：9

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