Argininyl-fructosyl-glucose (AFG) antagonizes D-galactose-induced cellular senescence of neuro-2a via inhibiting endoplasmic reticulum stress and apoptosis

被引：1
作者：
Tang, Shan [1 ,2 ,3 ]
Hou, Yun-yi [1 ]
Hu, Rui-yi [1 ]
Zhang, Jun-jie [1 ]
Gao, Xu-fei [1 ]
Zhang, Ming [4 ]
Wang, Zi [1 ,3 ]
Zhang, Rui [1 ]
Jia, Pin-hui [1 ,3 ]
Li, Wei [1 ,2 ,3 ]
机构：
[1] Jilin Agr Univ, Coll Chinese Med Mat, Changchun 130118, Peoples R China
[2] Jilin Agr Univ, Coll Life Sci, Engn Res Ctr, Chinese Minist Educ Bioreactor & Pharmaceut Dev, Changchun 130118, Peoples R China
[3] Jilin Prov Int Joint Res Ctr Dev & Utilizat Authen, Changchun 130118, Peoples R China
[4] Jilin Univ, Coll Med, Changchun 130021, Peoples R China
来源：
JOURNAL OF FUNCTIONAL FOODS | 2024年 / 115卷
关键词：
Argininyl-fructosyl-glucose; Ginseng; D; -galactose; N2a cell; Senescence; Endoplasmic reticulum stress; Apoptosis; OXIDATIVE STRESS; ACTIVATION; PRODUCTS; GINSENG; BRAIN;
D O I：
10.1016/j.jff.2024.106102
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Argininyl-fructosyl-glucose (AFG), a distinctive non-saponin compound isolated from red ginseng (Panax ginseng. C.A. Meyer), demonstrates neuroprotective effects. However, the molecular mechanism underlying AFG's antiaging properties remains unclear. This study established an in vitro D-galactose-induced N2a cells subacute aging model to investigate AFG's anti-aging activity and potential mechanisms. Results indicated that AFG treatment (8 mu M) significantly reduces the overexpression of aging-related proteins (p53/p21/p16) induced by D-galactose, highlighting its anti-aging efficacy. AFG concurrently diminishes ROS accumulation, lowering extracellular lactate dehydrogenase and alleviating intracellular oxidative stress. Notably, AFG suppresses the PERK/CHOP/caspase 12 pathway, reducing intracellular Ca2+ concentration and mitigating endoplasmic reticulum (ER) stress. Additionally, AFG decreases Bax and caspase 3 expression, elevates Bcl-2 and mitochondrial membrane potential, mitigating apoptosis. NAC with 4-PBA validated the results. Overall, AFG protects N2a cells from aging by reducing ER stress and apoptosis, suggesting its potential as a key substance for treating aging and neurodegenerative diseases.
引用
收藏
页数：15
相关论文
共 46 条
[1]   Endoplasmic Reticulum (ER) Stress Response Failure in Diseases [J].
Bhattarai, Kashi Raj ;
Chaudhary, Manoj ;
Kim, Hyung-Ryong ;
Chae, Han-Jung .
TRENDS IN CELL BIOLOGY, 2020, 30 (09) :672-675
[2]   Endoplasmic Reticulum Stress and Oxidative Stress in Cell Fate Decision and Human Disease [J].
Cao, Stewart Siyan ;
Kaufman, Randal J. .
ANTIOXIDANTS & REDOX SIGNALING, 2014, 21 (03) :396-413
[3]   Oxidation scrutiny in persuaded aging and chronological aging at systemic redox homeostasis level [J].
Cebe, Tamer ;
Atukeren, Pinar ;
Yanar, Karolin ;
Kuruc, Aylin Irmak ;
Ozan, Tuna ;
Kunbaz, Ahmad ;
Sitar, Mustafa Erinc ;
Mirmaroufizibandeh, Reza ;
Aydin, Seval ;
Cakatay, Ufuk .
EXPERIMENTAL GERONTOLOGY, 2014, 57 :132-140
[4]   Presynaptic store-operated Ca2+ entry drives excitatory spontaneous neurotransmission and augments endoplasmic reticulum stress [J].
Chanaday, Natali L. ;
Nosyreva, Elena ;
Shin, Ok-Ho ;
Zhang, Hua ;
Aklan, Iltan ;
Atasoy, Deniz ;
Bezprozvanny, Ilya ;
Kavalali, Ege T. .
NEURON, 2021, 109 (08) :1314-+
[5]   The effects of agomelatine on endoplasmic reticulum stress related to mitochondrial dysfunction in hippocampus of aging rat model [J].
Chanmanee, Teera ;
Wongpun, Jittiporn ;
Tocharus, Chainarong ;
Govitrapong, Piyarat ;
Tocharus, Jiraporn .
CHEMICO-BIOLOGICAL INTERACTIONS, 2022, 351
[6]   Original Metabolomics investigation on antiobesity effects of Corydalis bungeana on high-fat high-sugar diet-induced obese rats [J].
Fu, Minghai ;
Bao, Terigele ;
Yu, Hongzhen ;
LiSha, A. ;
Li, HuiFang ;
Ba, Genna ;
Cho, Sungbo .
CHINESE HERBAL MEDICINES, 2022, 14 (03) :414-421
[7]   Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018 [J].
Galluzzi, Lorenzo ;
Vitale, Ilio ;
Aaronson, Stuart A. ;
Abrams, John M. ;
Adam, Dieter ;
Agostinis, Patrizia ;
Alnemri, Emad S. ;
Altucci, Lucia ;
Amelio, Ivano ;
Andrews, David W. ;
Annicchiarico-Petruzzelli, Margherita ;
Antonov, Alexey V. ;
Arama, Eli ;
Baehrecke, Eric H. ;
Barlev, Nickolai A. ;
Bazan, Nicolas G. ;
Bernassola, Francesca ;
Bertrand, Mathieu J. M. ;
Bianchi, Katiuscia ;
Blagosklonny, Mikhail V. ;
Blomgren, Klas ;
Borner, Christoph ;
Boya, Patricia ;
Brenner, Catherine ;
Campanella, Michelangelo ;
Candi, Eleonora ;
Carmona-Gutierrez, Didac ;
Cecconi, Francesco ;
Chan, Francis K. -M. ;
Chandel, Navdeep S. ;
Cheng, Emily H. ;
Chipuk, Jerry E. ;
Cidlowski, John A. ;
Ciechanover, Aaron ;
Cohen, Gerald M. ;
Conrad, Marcus ;
Cubillos-Ruiz, Juan R. ;
Czabotar, Peter E. ;
D'Angiolella, Vincenzo ;
Dawson, Ted M. ;
Dawson, Valina L. ;
De laurenzi, Vincenzo ;
De Maria, Ruggero ;
Debatin, Klaus-Michael ;
DeBerardinis, Ralph J. ;
Deshmukh, Mohanish ;
Di Daniele, Nicola ;
Di Virgilio, Francesco ;
Dixit, Vishva M. ;
Dixon, Scott J. .
CELL DEATH AND DIFFERENTIATION, 2018, 25 (03) :486-541
[8]   Folic acid prevents habituation memory impairment and oxidative stress in an aging model induced by D-galactose [J].
Garcez, Michelle Lima ;
Cassoma, Ricardo Chiengo Sapalo ;
Mina, Francielle ;
Bellettini-Santos, Tatiani ;
da Luz, Aline Pereira ;
Schiavo, Gustavo Luis ;
Medeiros, Eduarda Behenck ;
Campos, Ana Carolina Brunatto Falchetti ;
da Silva, Sabrina ;
Rempel, Lisienny Campoli Tono ;
Steckert, Amanda Valnier ;
Barichello, Tatiana ;
Budni, Josiane .
METABOLIC BRAIN DISEASE, 2021, 36 (02) :213-224
[9]   Cellular senescence regulated by SWI/SNF complex subunits through p53/p21 and p16/pRB pathway [J].
He, Ling ;
Chen, Ying ;
Feng, Jianguo ;
Sun, Weichao ;
Li, Shun ;
Ou, Mengting ;
Tang, Liling .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2017, 90 :29-37
[10]   The mitochondria-targeting antioxidant MitoQ alleviated lipopolysaccharide/D-galactosamine-induced acute liver injury in mice [J].
Hu, Kai ;
Xiao, Lidan ;
Li, Longjiang ;
Shen, Yi ;
Yang, Yongqiang ;
Huang, Jiayi ;
Wang, Yaping ;
Zhang, Li ;
Wen, Sha ;
Tang, Li .
IMMUNOLOGY LETTERS, 2021, 240 :24-30
12345