Engineering an upconversion fluorescence sensing platform with "off-on" pattern through specific DNAzyme-mediated signal amplification for supersensitive detection of uranyl ion

被引：2

作者：

Zhang, Xinyu ^{[1
]}

Wang, Yue ^{[1
]}

Gong, Mi ^{[1
]}

Xiong, Lihao ^{[1
]}

Song, Jiayi ^{[1
]}

Chen, Sihan ^{[1
]}

Tong, Yuqi ^{[1
]}

Liu, Yu ^{[2
]}

Li, Le ^{[1
]}

Zhen, Deshuai ^{[1
,2
]}

机构：

[1] Univ South China, Coll Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China

[2] Hunan Univ, State Key Lab Chemo Biosensing & Chem Engn, Changsha 410082, Peoples R China

来源：

MICROCHIMICA ACTA | 2024年 / 191卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Upconversion nanoparticles; Fluorescence resonance energy transfer; Uranyl; Deoxyribozyme; Hybrid chain reaction; COLORIMETRIC DETECTION; GOLD; URANIUM; DNA;

D O I：

10.1007/s00604-024-06559-y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An upconversion fluorescence sensing platform was developed with upconversion nanoparticles (UCNPs) as energy donors and gold nanoparticles (AuNPs) as energy acceptors, based on the FRET principle. They were used for quantitative detection of uranyl ions (UO22+) by amplifying the signal of the hybrid chain reaction (HCR). When UO22+ are introduced, the FRET between AuNPs and UCNPs can be modulated through a HCR in the presence of high concentrations of sodium chloride. This platform provides exceptional sensitivity, with a detection limit as low as 68 pM for UO22+ recognition. We have successfully validated the reliability of this method by analyzing authentic water samples, achieving satisfactory recoveries (89.00%-112.50%) that are comparable to those of ICP-MS. These results indicate that the developed sensing platform has the capability to identify trace UO22+ in complex environmental samples.

引用

页数：11