Antivirulence Effects of Trans-Resveratrol and Curcumin on Methicillin-Resistant Staphylococcus aureus (MRSA) from Saudi Arabia

被引:7
作者
Alqahtani, Maisa [1 ]
Almukainzi, May [2 ]
Alghoribi, Majed F. [3 ,4 ,5 ]
El-Mahdy, Areej M. [6 ]
机构
[1] Princess Nourah bint Abdulrahman Univ, Coll Sci, Biol Dept, Riyadh 11671, Saudi Arabia
[2] Princess Nourah bint Abdulrahman Univ, Coll Pharm, Dept Pharmaceut Sci, POB 84428, Riyadh 11671, Saudi Arabia
[3] King Abdullah Int Med Res Ctr, Infect Dis Res Dept, Riyadh 11426, Saudi Arabia
[4] King Abdulaziz Med City KAMC, Minist Natl Guard Hlth Affairs MNGHA, Dept Pathol & Lab Med, Riyadh 11481, Saudi Arabia
[5] King Saud bin Abdulaziz Univ Hlth Sci, Dept Pathol & Lab Med, Riyadh 11426, Saudi Arabia
[6] Mansoura Univ, Fac Pharm, Dept Microbiol & Immunol, Mansoura 35516, Egypt
来源
LIFE-BASEL | 2024年 / 14卷 / 04期
关键词
phenolic compounds; virulence factors; biofilm; hemolysin; protease; lecithinase; VIRULENCE FACTORS; IN-VITRO; ANTIBACTERIAL; BIOFILM; PLANT; PROTEASE; LONGA;
D O I
10.3390/life14040491
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Methicillin-resistant Staphylococcus aureus (MRSA) is a common resistant bacterium, whose resistance has expanded to commonly used antibiotics. It is crucial to create novel treatments to tackle bacterial resistance. Trans-resveratrol and curcumin are naturally occurring phenolic compounds, whose effects on MRSA virulence are the subject of this investigation. Sub-MICs of trans-resveratrol and curcumin were tested on the virulence factors of 50 MRSA clinical isolates (CIs), including biofilm, hemolysin, hemagglutination, protease, and lecithinase. The distribution of the virulence factors of the CIs was as follows: hemolysin: 98%, hemagglutination: 70%, protease: 62%, biofilm: 56%, and lecithinase: 52%. The sub-MIC that could reduce the effect of the tested virulence factors by 50% or more (IC50) was observed in the strains that showed susceptibility to the individual administration of trans-resveratrol at 50 mu g/mL and curcumin at 20 mu g/mL. Hemagglutination and hemolysin activity were inhibited by at least 50% in the majority of CIs (57-94%). Meanwhile, the IC50 for protease and biofilm was observed in 6.5-17.8% of the CIs. A few of the CIs were susceptible to lecithinase inhibition, but all showed a full inhibition. This research supports the possibility of the use of these compounds to reduce the bacterial virulence that can reduce antibiotic utilization, and eventually, they can become a potential alternative treatment in combating bacterial resistance.
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页数:14
相关论文
共 44 条
[1]  
Aggarwal BB, 2007, ADV EXP MED BIOL, V595, P1
[2]  
Anacarso I., 2013, Univers. J. Microbiol. Res, V1, P1, DOI [10.13189/ujmr.2013.010101, DOI 10.13189/UJMR.2013.010101]
[3]  
[Anonymous], 1995, Water Microbiology-Heterotrophic Colony Count Methods-Pour Plate Method using Plate Count Agar
[4]   Biological activities of Curcuma longa L. [J].
Araújo, CAC ;
Leon, LL .
MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2001, 96 (05) :723-728
[5]   Therapeutic potential of resveratrol:: the in vivo evidence [J].
Baur, Joseph A. ;
Sinclair, David A. .
NATURE REVIEWS DRUG DISCOVERY, 2006, 5 (06) :493-506
[6]  
Biswajit Batabyal Biswajit Batabyal, 2012, International Research Journal of Biological Sciences, V1, P65
[7]   Red wines and flavonoids diminish Staphylococcus aureus virulence with anti-biofilm and anti-hemolytic activities [J].
Cho, Hyun Seob ;
Lee, Jin-Hyung ;
Cho, Moo Hwan ;
Lee, Jintae .
BIOFOULING, 2015, 31 (01) :1-11
[8]   Resveratrol Nanoparticles: A Promising Therapeutic Advancement over Native Resveratrol [J].
Chung, Ill-Min ;
Subramanian, Umadevi ;
Thirupathi, Prabhu ;
Venkidasamy, Baskar ;
Samynathan, Ramkumar ;
Gangadhar, Baniekal Hiremath ;
Rajakumar, Govindasamy ;
Thiruvengadam, Muthu .
PROCESSES, 2020, 8 (04)
[9]   Methicillin-resistant Staphylococcus aureus (MRSA): antibiotic-resistance and the biofilm phenotype [J].
Craft, Kelly M. ;
Nguyen, Johny M. ;
Berg, Lawrence J. ;
Townsend, Steven D. .
MEDCHEMCOMM, 2019, 10 (08) :1231-1241
[10]   Blue light emitting diodes enhance the antivirulence effects of Curcumin against Helicobacter pylori [J].
Darmani, Homa ;
Smadi, Ehda A. M. ;
Bataineh, Sereen M. B. .
JOURNAL OF MEDICAL MICROBIOLOGY, 2020, 69 (04) :617-624