Jasmonates Promote β-Amylase-Mediated Starch Degradation to Confer Cold Tolerance in Tomato Plants

被引:6
作者
Fan, Xiulan [1 ]
Lin, Huanru [1 ]
Ding, Fei [1 ]
Wang, Meiling [1 ]
机构
[1] Liaocheng Univ, Sch Life Sci, Liaocheng 252000, Peoples R China
来源
PLANTS-BASEL | 2024年 / 13卷 / 08期
关键词
JA; MYC2; beta-amylase; cold stress; tomato; TRANSCRIPTION FACTOR; SOLUBLE SUGARS; OXIDATIVE STRESS; ACCUMULATION; TEMPERATURE; GENE; INDUCTION; RESPONSES; IMPROVES; LEAVES;
D O I
10.3390/plants13081055
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cold stress severely restricts growth and development, reduces yields, and impairs quality in tomatoes (Solanum lycopersicum). Amylase-associated starch degradation and soluble sugar accumulation have been implicated in adaptation and resistance to abiotic stress. Here, we report a beta-amylase (BAM) gene, SlBAM3, which plays a central role in tomato cold tolerance. The expression of SlBAM3 was triggered by cold stress. SlBAM3 knockout using the CRISPR/Cas9 system retarded starch degradation and reduced soluble sugar accumulation in tomato plants, eventually attenuating cold tolerance. Expression analysis revealed that the SlBAM3 transcript level was boosted by MeJA. Furthermore, MYC2, an essential component of the JA signaling pathway, could bind to the SlBAM3 promoter and directly activate SlBAM3 transcription, as revealed by yeast one-hybrid and dual LUC assays. In addition, the suppression of MYC2 resulted in increased starch accumulation, decreased soluble sugar content, and reduced tolerance to cold stress in tomato plants. Taken together, these findings demonstrate that JA positively regulates beta-amylase-associated starch degradation through the MYC2-SlBAM3 module in tomato during cold stress. The results of the present work expand our understanding of the mechanisms underlying BAM gene activation and starch catabolism under cold stress. The regulatory module of SlBAM3 can be further utilized to breed tomato cultivars with enhanced cold tolerance.
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页数:14
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共 32 条
[21]   Cold-regulated protein (SlCOR413IM1) confers chilling stress tolerance in tomato plants [J].
Ma, Xiaocui ;
Chen, Chong ;
Yang, Minmin ;
Dong, Xinchun ;
Lv, Wei ;
Meng, Qingwei .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2018, 124 :29-39
[22]   SlMPK1-and SlMPK2-mediated SlBBX17 phosphorylation positively regulates CBF-dependent cold tolerance in tomato [J].
Song, Jianing ;
Lin, Rui ;
Tang, Mingjia ;
Wang, Lingyu ;
Fan, Pengxiang ;
Xia, Xiaojian ;
Yu, Jingquan ;
Zhou, Yanhong .
NEW PHYTOLOGIST, 2023, 239 (05) :1887-1902
[23]   SlMAPK3 Positively Regulates the Ethylene Production of Postharvest Tomato Fruits and Is Involved in Ethylene-Mediated Cold Tolerance [J].
Shu, Pan ;
Li, Yujing ;
Sheng, Jiping ;
Shen, Lin .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2023, 71 (15)
[24]   Enhancement in Seed Priming-Induced Starch Degradation of Rice Seed Under Chilling Stress via GA-Mediated α-Amylase Expression [J].
Nie, Lixiao ;
Song, Shaokun ;
Yin, Qi ;
Zhao, Tingcheng ;
Liu, Hongyan ;
He, Aibin ;
Wang, Weiqin .
RICE, 2022, 15 (01)
[25]   Heterotrimeric G-protein α subunit (LeGPA1) confers cold stress tolerance to processing tomato plants (Lycopersicon esculentumMill) [J].
Guo, Xinyong ;
Li, Juju ;
Zhang, Li ;
Zhang, Zhanwen ;
He, Ping ;
Wang, Wenwen ;
Wang, Mei ;
Wang, Aiying ;
Zhu, Jianbo .
BMC PLANT BIOLOGY, 2020, 20 (01)
[26]   H2O2 and NO are involved in trehalose-regulated oxidative stress tolerance in cold-stressed tomato plants [J].
Liu, Tao ;
Ye, Xueling ;
Li, Meng ;
Li, Jianming ;
Qi, Hongyan ;
Hu, Xiaohui .
ENVIRONMENTAL AND EXPERIMENTAL BOTANY, 2020, 171
[27]   Sesquiterpene glucosylation mediated by glucosyltransferase UGT91Q2 is involved in the modulation of cold stress tolerance in tea plants [J].
Zhao, Mingyue ;
Zhang, Na ;
Gao, Ting ;
Jin, Jieyang ;
Jing, Tingting ;
Wang, Jingming ;
Wu, Yi ;
Wan, Xiaochun ;
Schwab, Wilfried ;
Song, Chuankui .
NEW PHYTOLOGIST, 2020, 226 (02) :362-372
[28]   SEC1-C3H39 module fine-tunes cold tolerance by mediating its target mRNA degradation in tomato [J].
Xu, Jin ;
Huang, Zelan ;
Du, Hongyu ;
Tang, Mingjia ;
Fan, Pengxiang ;
Yu, Jingquan ;
Zhou, Yanhong .
NEW PHYTOLOGIST, 2023, 237 (03) :870-884
[29]   Phytochromes enhance SOS2-mediated PIF1 and PIF3 phosphorylation and degradation to promote Arabidopsis salt tolerance [J].
Ma, Liang ;
Han, Run ;
Yang, Yongqing ;
Liu, Xiangning ;
Li, Hong ;
Zhao, Xiaoyun ;
Li, Jianfang ;
Fu, Haiqi ;
Huo, Yandan ;
Sun, Liping ;
Yan, Yan ;
Zhang, Hongyan ;
Li, Zhen ;
Tian, Feng ;
Li, Jigang ;
Guo, Yan .
PLANT CELL, 2023, 35 (08) :2997-3020
[30]   Genetic Improvement of Tomato (Solanum lycopersicum) with AtDREB1A Gene for Cold Stress Tolerance using Optimized Agrobacterium-mediated Transformation System [J].
Shah, Sabir Hussain ;
Ali, Shaukat ;
Hussain, Zahid ;
Jan, Sohail Ahmad ;
Jalal-ud-Din ;
Ali, Ghulam Muhammad .
INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, 2016, 18 (03) :471-482