Extended-spectrum beta-lactamases in clinical isolates of Escherichia coli and Klebsiella pneumoniae recovered from patients at the Tamale Teaching Hospital, Ghana

被引:1
作者
Tetteh, Francis Kwame Morgan [1 ,2 ]
Ablordey, Anthony [3 ]
Obeng-Nkrumah, Noah [1 ]
Opintan, Japheth Awuletey [1 ]
机构
[1] Univ Ghana, Med Sch, Dept Med Microbiol, Accra, Ghana
[2] 37 Mil Hosp, Pathol Div, Accra, Ghana
[3] Univ Ghana, Noguchi Mem Inst Med Res, Dept Bacteriol, Accra, Ghana
来源
PLOS ONE | 2024年 / 19卷 / 04期
关键词
GRAM-NEGATIVE BACTERIA; CTX-M; RISK-FACTORS; RESISTANCE; EMERGENCE; EPIDEMIOLOGY; SPREAD; KUMASI;
D O I
10.1371/journal.pone.0300596
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Introduction Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae are pathogens of significant public health interest for which new antibiotics are urgently needed. Aim To determine the prevalence of ESBLs in E. coli and K. pneumoniae isolates from patients attending the Tamale Teaching Hospital (TTH) in Ghana. Methodology The study was a cross-sectional study involving convenience sampling of E. coli and K. pneumoniae isolates from consenting patients' clinical specimens, between April and June 2015. Antimicrobial susceptibility test was performed, and ESBL-producer phenotypes were further screened for Bla(TEM), Bla(SHV), and Bla(CTX-M) genes. Patients' clinical data were additionally collected using a structured questionnaire. Results Of the 150 non-duplicate E. coli and K. pneumoniae isolates identified, 140 were confirmed as E. coli (84%, n = 117) and K. pneumoniae (16%, n = 23). Of these, sixty-two (44%) [E. coli (84%; n = 52); K. pneumoniae (16%; n = 10)] phenotypically expressed ESBLs. The proportion of ESBL-producing isolates was higher in adults (15-65 years) than in neonates (< 28 days) (p = 0.14). Most of the isolates showed a high percentage resistance to ampicillin (96%) and tetracycline (89%), but a relatively lower resistance to amikacin (36%). No isolate was resistant to meropenem. More ESBL producers were multidrug resistant compared to non-ESBL-producers [23% (14/62) versus 18% (14/78); p = 0.573]. Overall, 74% (n = 46) of the ESBL genotypes expressed Bla(CTX-M-1) genes, followed by 63% (n = 39) Bla(TEM), and 16% (n = 10) Bla(SHV). The study showed a high prevalence of ESBL-positive E. coli and K. pneumoniae, mostly CTX-M-1 producers at TTH. Conclusion Routine laboratory ESBL screening is warranted to inform patient management.
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