Imidacloprid: Impact on Africanized Apis mellifera L. (Hymenoptera: Apidae) workers and honey contamination

被引：6

作者：

Paloschi C.L. ^{[1
]}

Tavares M.H.F. ^{[1
]}

Berte E.A. ^{[2
]}

Model K. ^{[1
]}

Rosa K.M. ^{[1
]}

Conceição F.G.D. ^{[2
]}

Domanski F.R. ^{[3
]}

de Souza Vismara E. ^{[2
]}

Montanher P.F. ^{[2
]}

Maciel R.M.A. ^{[2
]}

Ribeiro L.D.S. ^{[2
]}

Ramos Mertz N. ^{[2
]}

Sampaio S.C. ^{[1
]}

Costa F.M. ^{[2
]}

Lozano E.R. ^{[2
]}

Potrich M. ^{[2
]}

机构：

[1] Universidade Estadual do Oeste do Paraná, Paraná, Cascavel

[2] Universidade Tecnológica Federal do Paraná, Paraná, Dois Vizinhos

[3] Universidade Estadual de Maringá, Paraná, Maringá

来源：

Chemosphere | 2023年 / 338卷

关键词：

Bee; Neonicotinoids; Selectivity; Toxicity;

D O I：

10.1016/j.chemosphere.2023.139591

中图分类号：

学科分类号：

摘要：

Apis mellifera L. (Hymenoptera: Apidae) is fundamental in the production chain, ensuring food diversity through the ecosystem service of pollination. The aim of this work was to evaluate the impact of imidacloprid, orally, topically, and by contact, on A. mellifera workers and to verify the presence of this active ingredient in honey. Toxicity levels were verified by bioassays. In bioassay 1, the levels correspond to the percentages of 100, 10, 1, 0.1, and 0.01% of the recommended concentration for field application of the commercial product Nortox® (active ingredient imidacloprid), with which we obtained the mean lethal concentration (LC50) in 48 h for A. mellifera, determining the concentration ranges to be used in the subsequent bioassays. Bioassays 2 and 3 followed the guidelines of the Organization for Economic Cooperation and Development, which specify the LC50 (48 h). In bioassay 4, the LC50 (48 h) and the survival rate of bees for a period of 120 h were determined by contact with a surface contaminated with imidacloprid, and in bioassay 5, the interference of the insecticide with the flight behavior of bees was evaluated. Honey samples were collected in agroecological and conventional georeferenced apiaries and traces of the imidacloprid were detected by means of high-performance liquid chromatography (HPLC-UV) with extraction by SPE C18. Bee survival was directly affected by the concentration and exposure time, as well behavioral performance, demonstrating the residual effect of imidacloprid on A. mellifera workers. Honey samples from a conventional apiary showed detection above the maximum residue limits (MRL) allowed by the European Union (0.05 μg mL−1), but samples from other apiaries showed no traces of this insecticide. Imidacloprid affects the survival rate and behavior of Africanized A. mellifera and honey quality. © 2023 Elsevier Ltd

引用

共 88 条

[1] Abati R., Sampaio A.R., Maciel R.M.A., Colombo F.C., Libardoni G., Battisti L., Lozano E.R., Ghisi N.C., Costa-Maia F.M., Potrich M., Bees and pesticides: the research impact and scientometrics relations, Environ. Sci. Pollut. Control Ser., 28, pp. 32282-32298, (2021)
[2] Aliferis K.A., Tarantilis P.A., Harizanis P.C., Alissandrakis E., Botanical discrimination and classification of honey bee samples applying gas chromatography/mass spectrometry fingerprinting of headspace volatile compounds, Food Chem., 121, pp. 856-862, (2010)
[3] Al-Waili N., Salom K., Al-Ghamdi A., Ansari M.J.E.N., Scozzafava A., Tan D.X., Videla L.A., Antibiotic, pesticide, and microbial contaminants of honey bee: human health hazards, Sci. World J., 9, (2012)
[4] Amaro P., Godinho J., Pesticidas e abelhas, Rev. Ciencias Agrar., 5, pp. 53-62, (2012)
[5] Resolution RE N<sup>o</sup>, 166, (2017)
[6] Baptista A.P.M., Carvalho G.A., Carvalho S.M., Carvalho C.F., Bueno Filho J.S.S., Toxicity of pesticides used in citrus crop to Apis mellifera, Ciência Rural., 39, pp. 955-961, (2009)
[7] Bonmatin J.M., Giorio C., Girolami V., Goulson D., Kreutzweiser D.P., Krupke C., Liess M., Long E., Marzaro M., Mitchell E.A., Noome D.A., Simon-Delso N., Tapparo A., Environmental fate and exposure
[8] neonicotinoids and fipronil, Environ. Sci. Pollut. Control Ser., 22, pp. 35-67, (2015)
[9] Normative Instruction SDA n 13, de 15 de Julho de 2015, (2015)
[10] Biddinger D.J., Robertson J.L., Mullin C., Frazier J., Ashcraft S.A., Rajotte E.G., Joshi N.K., Vaughn M., Comparative toxicities and synergism of apple orchard pesticides to Apis mellifera (L.) and Osmia cornifrons (Radoszkowski), PLoS One, 8, (2013)

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