Simulation and Experimental Study on Preferential Killing of Chemoresistance Tumor Cells Induced by the High-Frequency Permeation Effect of Nanosecond Pulse Field

被引：0
|
作者：
Liu H. [1 ]
Dong S. [1 ]
Ning J. [1 ]
Zheng S. [1 ]
Yao C. [1 ]
机构：
[1] State Key Laboratory of Power Transmission Equipment & System Security and New Technology, Chongqing University, Chongqing
来源：
Diangong Jishu Xuebao/Transactions of China Electrotechnical Society | 2019年 / 34卷 / 22期
关键词：
Chemoresistance; Electroporation; Intracellular electromainpulation; Nanosecond pulse; Selectivity; Targeted ablation; Tumor treatment;
D O I：
10.19595/j.cnki.1000-6753.tces.181456
中图分类号：
学科分类号：
摘要：
In order to overcome the barrier of clinical tumor treatment-chemoresistance, and to make up for the blank of bioelectric effect on chemoresistance tumor cells (CRTCs) induced by nanosecond pulse electric fields (nsPEFs), simulations and experiments were combined in this paper. Firstly, based on the finite element platform, a five-layer dielectric model considering organelles and a numerical model considering the electroporation of inner and outer membranes were established. The high-frequency permeation effect, targeting characteristics and the influence from cell nucleus size were analyzed. Then, taking A549 and homologous A549/R as research objects, this paper further studied the fluorescence dissipation of inner/outer membrane under nsPEFs, and compared the differences of the morphology and killing effects between A549 and A549/R based on the fluorescent probe and flow techniques. It is shown that nsPEFs can efficiently target the inner membrane and induce the fluorescence dissipation of the cell nuclear membrane. This intracellular effect is positively correlated with the nucleus size (or nucleo-cytoplasmic ratio, NCR), causing higher killing effect on A549/R with higher NCR. Therefore, high voltage nsPEFs can target inner membrane to priority kill CRTCs. These results provide theoretical and experimental basis for selective killing of the CRTCs, and also lay a foundation for nsPEFs as a new physical selective method, which can be combined with other tumor treatment methods to achieve effective ablation of refractory tumor tissues. © 2019, Electrical Technology Press Co. Ltd. All right reserved.
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页码：4839 / 4848
页数：9
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