Diversity and Predictive Functional of Caragana jubata Bacterial Community in Rhizosphere and Non-rhizosphere Soil at Different Altitudes

被引：4

作者：

Li Y.-Y. ^{[1
,2
,3
]}

Xu T.-T. ^{[4
]}

Ai Z. ^{[3
]}

Wei L.-L. ^{[1
]}

Ma F. ^{[1
,2
]}

机构：

[1] School of Ecology and Environment, Ningxia University, Yinchuan

[2] Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China, Ningxia University, Yinchuan

[3] School of Geography and Planning, Ningxia University, Yinchuan

[4] School of Life Science, Ningxia University, Yinchuan

来源：

Huanjing Kexue/Environmental Science | 2023年 / 44卷 / 04期

关键词：

altitude; Caragana jubata; community structure; function prediction with PICRUSt; soil bacteria;

D O I：

10.13227/j.hjkx.202204165

中图分类号：

学科分类号：

摘要：

Caragana jubata, as the main dominant plant in the alpine and subalpine regions of northern China, is an important component of the local ecosystem. However, few studies have paid attention to its impact on the soil ecosystem and its response to environmental changes. Thus, in this study, we used high-throughput sequencing technology to investigate diversity and predictive function of rhizosphere and bulk soil bacteria communities of C. jubata from different altitudes. The results indicated that 43 phyla, 112 classes, 251 orders, 324 families, and 542 genera were obtained from the soil. The dominant phyla in all sample sites were Proteobacteria, Acidobacteria, and Actinobacteria. There were significant differences in bacterial diversity index and community structure between the rhizosphere and bulk soil at the same altitude, whereas the differences across altitudes were insignificant. PICRUSt analysis showed that the functional gene families were mainly related to 29 sub-functions, including amino acid metabolism, carbohydrate metabolism, and metabolism of cofactors and vitamins, and the abundance of metabolism was highest. There were significant correlations between the relative abundances of genes involved in the level II metabolic pathway of bacteria and phylum-level taxa, such as Proteobacteria, Acidobacteria, and Chloroflexi. The predicted functional compositions of soil bacteria also showed a significantly positive correlation with the dissimilarity in bacterial community structure, indicating that there was a strong relationship between bacterial community structure and functional genes. This study preliminarily discussed the characteristics and functional prediction analysis of bacterial communities in the rhizosphere and bulk soil of C. jubata at different altitudinal gradients, which provided data support for the ecological effects of constructive plants and their responses to environmental changes in high altitude areas. © 2023 Science Press. All rights reserved.

引用

页码：2304 / 2314

页数：10

共 48 条

[1]

Cui Y X, Fang L C, Guo X B, Et al., Responses of soil microbial communities to nutrient limitation in the desert-grassland ecological transition zone, Science of the Total Environment, 642, pp. 45-55, (2018)

[2]

Ma X, Luo Z Z, Zhang Y Q, Et al., Distribution characteristics and ecological function predictions of soil bacterial communities in rainfed alfalfa fields on the Loess Plateau, Acta PrataculturaeSinica, 30, 3, pp. 54-67, (2021)

[3]

Zhou X G, Gao D M, Liu J, Et al., Changes in rhizosphere soil microbial communities in a continuously monocropped cucumber (Cucumis sativus L.) system [ J], European Journal of Soil Biology, 60, pp. 1-8, (2014)

[4]

Nilsson R H, Anslan S, Bahram M, Et al., Mycobiome diversity: high-throughput sequencing and identification of fungi [ J ], Nature Reviews Microbiology, 17, 2, pp. 95-109, (2019)

[5]

ABhauer K P, Wemheuer B, Daniel R, Et al., Tax4 Fun: Predicting functional profiles from metagenomic 16S rRNA data, Bioinformatics, 31, 17, pp. 2882-2884, (2015)

[6]

Sun L J, Qi Y C, Dong Y S, Et al., Research progresses on the effects of global change on microbial community diversity of grassland soils [ J ], Progress in Geography, 31, 12, pp. 1715-1723, (2012)

[7]

Delgado-Baquerizo M, Fry E L, Eldridge D J, Et al., Plant attributes explain the distribution of soil microbial communities in two contrasting regions of the globe, New Phytologist, 219, 2, pp. 574-587, (2018)

[8]

Li N, Wang B R, An S S, Et al., Response of soil bacterial community structure to precipitation change in grassland of loess plateau, Environmental Science, 41, 9, pp. 4284-4293, (2020)

[9]

Shen C C, Gunina A, Luo Y, Et al., Contrasting patterns and drivers of soil bacterial and fungal diversity across a mountain gradient, Environmental Microbiology, 22, 9, pp. 3287-3301, (2020)

[10]

Li Y Q, Ma J W, Gao C, Et al., Denitrification rates and nirS-type denitrifying bacteria community structure characteristics of bulk and rhizosphere soil in spring and summer in the alpine wetlands of the Qinghai-Tibet Plateau, Environmental Science, 42, 10, pp. 4959-4967, (2021)

← 1 2 3 4 5 →