Features of Growth and Accumulation of Polyphenolic Compounds in Callus Cultures of Lavandula angustifolia Mill.

Characteristics of cell growth and the composition of polyphenolic compounds in callus cultures of Lavandula angustifolia Mill. grown on various nutrient media were determined. The addition of phytohormones to the nutrient medium is a productive biotechnological approach to regulation of secondary metabolite synthesis. The presence of alpha-naphthylacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), and kinetin in growth media was found to facilitate the accumulation of phenolic compounds (phenolic acids, flavonoids, and anthocyanins) in many cases. For each nutrient medium, growth curves were plotted and growth parameters were calculated. Analysis by means of the reverse phase HPLC combined with UV spectrophotometric detection revealed that rosmarinic acid is a major compound in all samples of lavender callus cultures. The patterns of rosmarinic acid accumulation were determined as a function of light availability and the composition of the nutrient medium. Among the four media examined, the medium containing 2.0 mg/L alpha-NAA and 0.1 mg/L BAP ensured the highest values of growth parameters and rosmarinic acid content (1.26 +/- 0.13%).