Digital PCR Improves Sensitivity and Quanti fi cation in Monitoring CAR-T Cells in B Cell Lymphoma Patients

被引:4
|
作者
de la Iglesia-San Sebastian, Ismael [1 ,2 ]
Carbonell, Diego [1 ,2 ]
Bastos-Oreiro, Mariana [1 ,2 ]
Perez-Corral, Ana [1 ,2 ]
Bailen, Rebeca [1 ,2 ]
Chicano, Maria [1 ,2 ]
Muniz, Paula [1 ,2 ]
Monsalvo, Silvia [1 ,2 ]
Escudero-Fernandez, Asuncion [1 ]
Oarbeascoa, Gillen [1 ,2 ]
Fernandez-Caldas, Paula [1 ,2 ]
Gomez-Centurion, Ignacio [1 ,2 ]
Pion, Marjorie [3 ]
Gayoso, Jorge [1 ,2 ]
Anguita, Javier [1 ,2 ]
Kwon, Mi [1 ,2 ]
Diez-Martin, Jose Luis [1 ,2 ,4 ]
Buno, Ismael [1 ,2 ,5 ]
Martinez-Laperche, Carolina [1 ,2 ,6 ]
机构
[1] Hosp Gen Univ Gregorio Maranon, Dept Hematol, Madrid, Spain
[2] Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
[3] Inst Invest Sanitaria Gregorio Maranon, Adv Immuno Regulat Grp, Hosp Gen Univ Gregorio Maranon, Madrid, Spain
[4] Univ Complutense Madrid, Dept Med, Madrid, Spain
[5] Genom Unit, Inst Invest Sanitaria Gregorio Maranon, Madrid, Spain
[6] Gregorio Maranon Gen Univ Hosp, Gregorio Maranon Hlth Res Inst IiSGM, Dept Hematol, C Doctor Esquerdo 46, Madrid 28007, Spain
来源
TRANSPLANTATION AND CELLULAR THERAPY | 2024年 / 30卷 / 03期
关键词
CAR-T cells; Digital PCR; Monitoring; Relapse; Sensitivity; Toxicity; THERAPY;
D O I
10.1016/j.jtct.2023.12.672
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Chimeric antigen receptor T cells (CAR-T) has emerged as a promising therapy, over 60% of patients fail to sustain a long-term response. The underlying factors that leads to the effectiveness of this therapy are not completely understood, CAR-T cell persistence and monitoring seems to be pivotal for ensuring a successful response. Various monitoring methods such as multiparametric flow cytometry (MFC) or quantitative PCR (qPCR) have been applied. Our objective is to develop digital PCR (dPCR) assays for detection and quanti fication of CAR-T cells, comparing them with MFC and qPCR. Samples taken at different follow-up times from 45 patients treated with CAR-T therapy were analyzed to assess the correlation between the different methodologies. dPCR presented a high correlation with MFC and qPCR (r = 0.97 and r = 0.87, respectively), while offering a higher sensitivity (0.01%) compared to MFC (0.1%) and qPCR (1%). dPCR emerged as an alternative and highly sensitivity method for monitoring CAR-T cell dynamics. This technique is well-suited for implementation in clinical practice as a complementary technique to MFC. (c) 2024 The American Society for Transplantation and Cellular Therapy. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
引用
收藏
页码:306e1 / 306e12
页数:12
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