Screening and Identification of Antagonistic Streptomyces for Biocontrol of Poplar Canker

被引：1

作者：

Chen Y. ^{[1
,2
]}

Liu Q. ^{[2
]}

Li L. ^{[2
]}

Zhang Y. ^{[2
]}

Han J. ^{[2
]}

Zhang Y. ^{[2
]}

机构：

[1] Key Laboratory of Forest Protection, National Forestry and Grassland Administration Research Institute of Forest Ecology, Environment and Protection, CAF, Beijing

[2] Jilin Provincial Academy of Forestry Sciences, Changchun

来源：

Linye Kexue/Scientia Silvae Sinicae | 2021年 / 57卷 / 07期

关键词：

Biological control; Poplar canker; Screening and identification; Streptomyces;

D O I：

10.11707/j.1001-7488.20210710

中图分类号：

学科分类号：

摘要：

【Objective】In order to control the poplar canker disease by biological control agent, the present study aimed to screen and obtain antagonistic strains from Streptomyces that can effectively inhibit poplar canker.【Method】A streptomyces strain with good biocontrol effect against the pathogen of poplar canker was obtained from the soil in the larch forest Hengshan Protection Station,Changbaishan Mountain National Nature Reserve, Jilin Province. The number was recorded as HS1. Multiple screening was used to determine the antibacterial activity of the strain and its fermentation broth; morphological, physiological and biochemical characteristics, 16S rDNA sequence analysis were used to determine its taxonomic status; artificial inoculation method was used to determine the preventive and therapeutic effects of HS1 fermentation broth on poplar canker.【Result】The result showed that strain HS1 and its fermentation broth had inhibitory effect on the tested plant pathogens, and the antibacterial spectrum was relatively wide. Among them, it had the strongest inhibitory effect on Botryosphaeria dothidea. The width of inhibition zone averagely was more than 20.66 mm and the diameter of the bacteriostatic circle of the fermentation broth reached 40.26 mm.The 16S rDNA sequence alignment analysis showed that the strain had the highest homology with Streptomyces scopuliridis; The result of artificial inoculation test showed that the preventive and therapeutic effects of strain HS1 fermentation broth on poplar canker were 60.87% and 71.74% respectirly.【Conclusion】The screened strain HS1 and its fermentation broth could effectively prevent and inhibit the occurrence and expansion of poplar canker disease, and also have a good antibacterial effect on other pathogens of many important agricultural and forest diseases. Therefore, it has a good for development and application prospects in biocontrol of forest pathogens. © 2021, Editorial Department of Scientia Silvae Sinicae. All right reserved.

引用

页码：92 / 100

页数：8

共 27 条

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