Development of a New Multispecies Protein A-Based ELISA for Serodiagnosis of Hepatitis E Infection with Validation in Domestic Swine

被引：0

作者：

Paz, Maria Gabriela Oliveira da ^{[1
]}

Bezerra, Matheus Filgueira ^{[1
]}

Mendes, Andrei Felix ^{[1
]}

da Silva, Adalucia ^{[1
]}

Lima, Gustavo Barbosa de ^{[1
]}

Gil, Laura Helena Vega Gonzales ^{[2
]}

Pena, Lindomar Jose ^{[2
]}

Diniz, George Tadeu Nunes ^{[3
]}

Drexler, Jan Felix ^{[4
,5
,6
]}

Oria, Reinaldo Barreto ^{[7
]}

Ferreira de Oliveira-Filho, Edmilson ^{[4
]}

Reis, Christian Robson de Souza ^{[1
]}

机构：

[1] Fundacao Oswaldo Cruz, Aggeu Magalhaes Inst, Dept Microbiol, Av Prof Moraes Rego S-N, BR-50740465 Recife, PE, Brazil

[2] Fundacao Oswaldo Cruz, Aggeu Magalhaes Inst, Dept Virol, Recife, Brazil

[3] Fundacao Oswaldo Cruz, Aggeu Magalhaes Inst, Stat & Geoproc Ctr, Recife, Brazil

[4] Charite Univ Med Berlin, Inst Virol, Berlin, Germany

[5] Free Univ Berlin, Berlin, Germany

[6] Humboldt Univ, Berlin, Germany

[7] Univ Fed Ceara, Sch Med, Lab Biol Tissue Healing Ontogeny & Nutr, Dept Morphol,Inst Biomed, Fortaleza, Brazil

来源：

FOODBORNE PATHOGENS AND DISEASE | 2024年 / 21卷 / 08期

关键词：

Hepatitis E; Zoonosis; diagnostic; seroprevalence; HEV;

D O I：

10.1089/fpd.2023.0179

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Hepatitis E virus (HEV) infects roughly 20 million people worldwide, causing self-limiting acute hepatic disease that can evolve into a chronic course. HEV-3, HEV-4, and HEV-7 genotypes are zoonotic and transmitted to humans by consuming raw or undercooked meat. Here, we developed an indirect ELISA based on the recombinant HEV-3 capsid and performed a seroprevalence study on domestic swine in northeastern Brazil. Our in-house ELISA was initially validated using a subset of 79 sera characterized by concordant results for two distinct commercial ELISA kits. Our ELISA exhibited excellent sensitivity (94%) and specificity (100%), with an area under the curve of 0.99 Further testing, including 212 swine sera, revealed a seroprevalence of 57.5% (95% confidence interval, 50.6-64.3%). Our findings indicate that the novel ELISA test could accurately detect specific anti-HEV antibodies in domestic pigs and should be further validated in humans and other mammals.

引用

页码：517 / 520

页数：4

共 10 条

[1] Evaluation of a multi-species Protein A-ELISA assay for plague serologic diagnosis in humans and other mammal hosts [J].

Bezerra, Matheus Filgueira ;

Xavier, Camila Cavalcanti ;

de Almeida, Alzira Maria Paiva ;

Reis, Christian Robson de Souza .

PLOS NEGLECTED TROPICAL DISEASES, 2022, 16 (05)

[2] Systematic Review of Hepatitis E Virus in Brazil: A One-Health Approach of the Human-Animal-Environment Triad [J].

da Silva Dias Moraes, Danny Franciele ;

Mesquita, Joao R. ;

Dutra, Valeria ;

Nascimento, Maria Sao Jose .

ANIMALS, 2021, 11 (08)

[3] Risk Analysis and Occurrence of Hepatitis E Virus (HEV) in Domestic Swine in Northeast Brazil [J].

de Oliveira-Filho, Edmilson F. ;

Lopes, Kennya G. S. ;

Cunha, Deivson S. ;

Silva, Virginia S. ;

Barbosa, Clara N. ;

Brandespim, Daniel F. ;

Pinheiro Junior, Jose Wilton ;

Bertani, Giovani R. ;

Gil, Laura H. V. G. .

FOOD AND ENVIRONMENTAL VIROLOGY, 2017, 9 (03) :256-259

[4] Uncovering neglected subtypes and zoonotic transmission of Hepatitis E virus (HEV) in Brazil [J].

dos Santos, Debora Regina Lopes ;

Duraes-Carvalho, Ricardo ;

Gardinali, Noemi Rovaris ;

Machado, Lais Ceschini ;

de Paula, Vanessa Salete ;

Wallau, Gabriel da Luz ;

de Oliveira, Jaqueline Mendes ;

Pena, Lindomar Jose ;

Pinto, Marcelo Alves ;

Gil, Laura Helena Vega Gonzalez ;

de Oliveira-Filho, Edmilson Ferreira .

VIROLOGY JOURNAL, 2023, 20 (01)

[5] Unraveling swine hepatitis E in the central region of Argentina through ELISA development and epidemiological insights [J].

Gutierrez, Silvina Elena ;

Arce, Lorena Paola ;

Bence, Angel Ricardo ;

Brancher, Julia Matias ;

Rivero, Mariana ;

Moran, Celeste ;

Vizoso-Pinto, Maria Guadalupe ;

Estein, Silvia Marcela .

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2023, 103

[6] In house ELISA based on recombinant ORF2 protein underline high prevalence of IgG anti-hepatitis E virus amongst blood donors in south Brazil [J].

Pandolfi, Rafael ;

de Almeida, Denise Ramos ;

Pinto, Marcelo Alves ;

Kreutz, Luiz Carlos ;

Frandoloso, Rafael .

PLOS ONE, 2017, 12 (05)

[7] A new recombinant F1 antigen as a cost and time-effective tool for plague diagnosis [J].

Tavares, Diego H. C. ;

Bezerra, Matheus F. ;

Magalhaes, Franklin B. ;

Cavalcanti, Thaise Y. V. L. ;

Xavier, Camila C. ;

Lear, Nilma C. ;

Almeida, Alzira M. P. ;

Reis, Christian R. S. .

JOURNAL OF MICROBIOLOGICAL METHODS, 2020, 172

[8] Hepatitis E virus: host tropism and zoonotic infection [J].

Wang, Bo ;

Meng, Xiang-Jin .

CURRENT OPINION IN MICROBIOLOGY, 2021, 59 :8-15

[9]

World Health Organization, Hepatitis E

[10]

Zhao CY, 2023, ADV EXP MED BIOL, V1417, P199, DOI 10.1007/978-981-99-1304-6_14

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