Extraction of Polysaccharide from Zingiber officinale Roscoe and Its Regulatory Effect on Intestinal Flora in Diabetic Mice

被引：0

作者：

Wang N. ^{[1
]}

Chen M. ^{[1
]}

Meng F. ^{[1
]}

Zhou L. ^{[1
]}

Lu Z. ^{[1
]}

机构：

[1] College of Food Science and Technology, Nanjing Agricultural University, Nanjing

来源：

Science and Technology of Food Industry | 2023年 / 44卷 / 04期

关键词：

antioxidant activity; diabetes; extraction; ginger polysaccharide; iintestinal flora;

D O I：

10.13386/j.issn1002-0306.2022060155

中图分类号：

学科分类号：

摘要：

Objectives: To investigate the regulatory effect of ginger polysaccharides on the intestinal flora of mice with type 2 diabetes (T2D) induced by the combination of a high-fat diet (HFD) and low-dose streptozotocin (STZ). Methods: Polysaccharides were extracted from ginger by hot water extraction and ethanol precipitation. Single-factor experiments and an orthogonal experiment were used to optimize the extraction conditions, and then the best deproteinization method was selected to obtain ginger polysaccharide (GP). The scavenging ability of GP to 2,2'-diazo-bis-3-ethylbenzothiazoline-6-sulfonic diammonium salt (ABTS+) free radicals and hydroxyl radicals evaluated its antioxidant capacity in vitro. Subsequently, a type 2 diabetes mouse model was established by a high-fat diet and STZ. The fasting blood glucose (FBG) concentration and glycated serum protein (GSP) level of mice in each group were measured. The composition of the intestinal flora of diabetic mice was analyzed by 16S rRNA high-throughput sequencing technique. Results: The optimum extraction process of GP was as follows: The ratio of material to water was 1:20 g/mL, the extraction temperature was 90 ℃, the extraction time was 1 h, and Sevag reagent was used 5 times to deproteinize to obtain GP, the yield was 2.91%±0.25%, of which the total sugar content was 43.44%±0.99%. At the concentration of 4 mg/mL in GP, the scavenging rates of ABTS+ free radical and hydroxyl free radical by GP were 66.09% and 65.73% respectively. After the intervention of GP, FBG concentration and GSP level in the diabetic mice were significantly reduced (P<0.05). The 16S rRNA sequencing results showed that GP altered the gut microbiota composition and relative abundance in diabetic mice, and the relative abundance of beneficial bacteria such as Adlercreutzia, Akkermansia and Lactobacillus increased, while the relative abundance of harmful bacteria such as Prevotella decreased. Conclusions: Ginger polysaccharides obtained by hot water extraction and Sevag deproteinization could alter the composition of the intestinal flora in diabetic mice. The results of this study would provide a theoretical basis for ginger polysaccharide in the prevention and treatment of T2D. © 2023, Editorial Department of Science and Technology of Food Science. All rights reserved.

引用

页码：278 / 286

页数：8

共 50 条

[21]

CHEN X H, WANG Z R, KAN J Q., Polysaccharides from ginger stems and leaves: Effects of dual and triple frequency ultrasound assisted extraction on structural characteristics and biological activities[J], Food Bioscience, 42, (2021)

[22]

FENG X, XIA Y, CHEN G T, Et al., Purification and structural analysis of polysaccharide from ginger peels[J], Food Science, 38, 6, (2017)

[23]

CHEN L J., Research on fast extraction and controlled degradation of polysaccharide from tremella, (2017)

[24]

WANG L W., Structural characterization of polysaccharide DOPA-1 isolated from Dendrobium officinale Kimura et Migo in Huoshan and its apoptosis effect on HepG2 cell[D], (2017)

[25]

YANG Y T., Study on ex-traction, purification and antioxidant activity of total flavonoids from Apocynum venetum[D], (2018)

[26]

LI S P., Study on extraction and anti-dementia effects of antioxidant constituents from ginger, (2015)

[27]

DUBOIS M, GILLES K A, HAMILTON J K, Et al., Colori-metric method for determination of sugars and related substances [J], Analytical Chemistry, 28, 3, (1956)

[28]

BRADFORD M M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the princi-ple of protein-dye binding[J], Analytical Biochemistry, 72, (1976)

[29]

LI C, HUANG Q, FU X, Et al., Characterization, antioxidant and immunomodulatory activities of polysaccharides from Prunella vulgaris Linn[J], International Journal of Biological Macromo-lecules, 75, pp. 298-305, (2015)

[30]

SHEN C., The separation and extraction, biological activities of polysaccharides from Shanxing rice wine and their effects on intestinal microflora[D], (2014)

← 1 2 3 4 5 →