Alginate- and Chitosan-Modified Gelatin Hydrogel Microbeads for Delivery of E. coli Phages

被引:10
作者
Moghtader, Farzaneh [1 ,2 ,3 ]
Solakoglu, Sencer [2 ]
Piskin, Erhan [1 ,3 ]
机构
[1] NanoBMT Nanobiyomedtek Biyomedikal & Biyoteknol Sa, TR-48800 Mugla, Turkiye
[2] Feyzciftligi AS, TR-16700 Bursa, Turkiye
[3] TiPHAE San Tic AS, Teknopark Istanbul, TR-34906 Istanbul, Turkiye
关键词
gelatin hydrogel microbeads; modifications; sodium alginate; chitosan coating; Escherichia coli; T4; phages; phage loading; release and storage stability; SODIUM ALGINATE; BACTERIOPHAGES; MICROENCAPSULATION; DEPOLYMERIZATION; THERAPY;
D O I
10.3390/gels10040244
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Bacterial infections are among the most significant health problems/concerns worldwide. A very critical concern is the rapidly increasing number of antibiotic-resistant bacteria, which requires much more effective countermeasures. As nature's antibacterial entities, bacteriophages shortly ("phages") are very important alternatives to antibiotics, having many superior features compared with antibiotics. The development of phage-carrying controlled-release formulations is still challenging due to the need to protect their activities in preparation, storage, and use, as well as the need to create more user-friendly forms by considering their application area/site/conditions. Here, we prepared gelatin hydrogel microbeads by a two-step process. Sodium alginate was included for modification within the initial recipes, and these composite microbeads were further coated with chitosan. Their swelling ratio, average diameters, and Zeta potentials were determined, and degradations in HCl were demonstrated. The target bacteria Escherichia coli (E.coli) and its specific phage (T4) were obtained from bacterial culture collections and propagated. Phages were loaded within the microbeads with a simple method. The phage release characteristics were investigated comparatively and were demonstrated here. High release rates were observed from the gelatin microbeads. It was possible to reduce the phage release rate using sodium alginate in the recipe and chitosan coating. Using these gelatin-based microbeads as phage carrier matrices-especially in lyophilized forms-significantly improved the phage stability even at room temperature. It was concluded that phage release from gelatin hydrogel microbeads could be further controlled by alginate and chitosan modifications and that user-friendly lyophilized phage formulations with a much longer shelf life could be produced.
引用
收藏
页数:17
相关论文
共 65 条
[1]   Bacteriophages reduce experimental contamination of hard surfaces, tomato, spinach, broccoli, and ground beef by Escherichia coli O157:H7 [J].
Abuladze, Tamar ;
Li, Manrong ;
Menetrez, Marc Y. ;
Dean, Timothy ;
Senecal, Andre ;
Sulakvelidze, Alexander .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (20) :6230-6238
[2]  
[Anonymous], 2021, Global Antimicrobial Resistance and Use Surveillance System (GLASS)
[3]   The resurgence of phage-based therapy in the era of increasing antibiotic resistance: From research progress to challenges and prospects [J].
Anyaegbunam, Ngozi Joannes ;
Anekpo, Chijioke Chinedu ;
Anyaegbunam, Zikora Kizito Glory ;
Doowuese, Yandev ;
Chinaka, Chinwe Blessing ;
Odo, Oluchi Joannes ;
Sharndama, Hyelnaya Cletus ;
Okeke, Onyekachi Philomena ;
Mba, Ifeanyi Elibe .
MICROBIOLOGICAL RESEARCH, 2022, 264
[4]   Bacteriophage T4 Nanoparticles as Materials in Sensor Applications: Variables That Influence Their Organization and Assembly on Surfaces [J].
Archer, Marie J. ;
Liu, Jinny L. .
SENSORS, 2009, 9 (08) :6298-6311
[5]   Phage on tap-a quick and efficient protocol for the preparation of bacteriophage laboratory stocks [J].
Bonilla, Natasha ;
Rojas, Maria Isabel ;
Cruz, Giuliano Netto Flores ;
Hung, Shr-Hau ;
Rohwer, Forest ;
Barr, Jeremy J. .
PEERJ, 2016, 4
[6]   Paving the way for phage therapy using novel drug delivery approaches [J].
Briot, Thomas ;
Kolenda, Camille ;
Ferry, Tristan ;
Medina, Mathieu ;
Laurent, Frederic ;
Leboucher, Gilles ;
Pirot, Fabrice .
JOURNAL OF CONTROLLED RELEASE, 2022, 347 :414-424
[7]   Photolytic depolymerization of alginate [J].
Burana-osot, Jankana ;
Hosoyama, Saori ;
Nagamoto, Yuzu ;
Suzuki, Sho ;
Linhardt, Robert J. ;
Toida, Toshihiko .
CARBOHYDRATE RESEARCH, 2009, 344 (15) :2023-2027
[8]   Effect of Experimental Parameters on Alginate/Chitosan Microparticles for BCG Encapsulation [J].
Caetano, Liliana A. ;
Almeida, Antonio J. ;
Goncalves, Lidia M. D. .
MARINE DRUGS, 2016, 14 (05)
[9]   Phage Therapy-History from Twort and d'Herelle Through Soviet Experience to Current Approaches [J].
Chanishvili, Nina .
ADVANCES IN VIRUS RESEARCH, VOL 83: BACTERIOPHAGES, PT B, 2012, 83 :3-40
[10]   Bacteriophage encapsulation: Trends and potential applications [J].
Choinska-Pulit, Anna ;
Mitula, Pawel ;
Sliwka, Paulina ;
Laba, Wojciech ;
Skaradzinska, Aneta .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 2015, 45 (02) :212-221