Microfluidic high-throughput 3D cell culture

被引：29

作者：

Ko, Jihoon ^{[1
]}

Park, Dohyun ^{[2
]}

Lee, Jungseub ^{[2
]}

Jung, Sangmin ^{[2
]}

Baek, Kyusuk ^{[3
]}

Sung, Kyung E. ^{[4
]}

Lee, Jeeyun ^{[5
]}

Jeon, Noo Li ^{[2
,3
,6
]}

机构：

[1] Gachon Univ, Dept BioNano Technol, Seongnam Si, South Korea

[2] Seoul Natl Univ, Mech Engn, Seoul, South Korea

[3] Qureator Inc, San Diego, CA 92121 USA

[4] US FDA, Ctr Biol Evaluat & Res, Cellular & Tissue Therapies Branch, Off Cellular Therapy & Human Tissue, Silver Spring, MD USA

[5] Sungkyunkwan Univ, Samsung Med Ctr, Dept Med, Div Hematol Oncol, Seoul, South Korea

[6] Seoul Natl Univ, Inst Adv Machines & Design, Seoul, South Korea

来源：

NATURE REVIEWS BIOENGINEERING | 2024年 / 2卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

ON-A-CHIP; PLURIPOTENT-STEM-CELL; INTERSTITIAL FLOW; ENDOTHELIAL-CELLS; PLATFORM; BARRIER; MEDICINE; STIMULATION; MATURATION; ORGANOIDS;

D O I：

10.1038/s44222-024-00163-8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

High-throughput 3D microfluidic cell culture systems can be designed to model aspects of human tissues and organs and may thus serve as non-clinical evaluation tools. They benefit from large-scale production, high throughput, compatibility with automated equipment, standardized analysis and the generation of physiologically relevant results. In this Review, we discuss how microfluidic devices can be designed with different biological complexity, cell sources and cell configurations, as well as physiological parameters to mimic human tissues. We examine standardization, scalability and automation strategies, and outline high-throughput data generation and analysis approaches to interpret readouts of microfluidic 3D cell culture models. Finally, we explore the potential of these tools as non-clinical testing systems for drug development and outline key future challenges in device design and application.

引用

页码：453 / 469

页数：17

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