Studies on Quality and Physicochemical Properties of Sea Cucumber Stichopus japonicus during Low Temperature Treatment

被引：0
作者：
He B. [1 ,2 ]
Feng D. [1 ,2 ]
Jiang D. [1 ,2 ]
Bai Y. [1 ]
Dong X. [1 ,2 ]
Qi H. [1 ,2 ]
机构：
[1] School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116034, Liaoning
[2] National Engineering Research Center of Seafood, Dalian, 116034, Liaoning
来源：
Journal of Chinese Institute of Food Science and Technology | 2019年 / 19卷 / 06期
关键词：
Caspases-3; Cathepsin L; Free radicals resonance (ESR); Low-temperature treatment; Sea cucumber; Texture;
D O I：
10.16429/j.1009-7848.2019.06.016
中图分类号：
学科分类号：
摘要：
In this study, sea cucumbers were as raw materials, treated at low temperature of 20℃, studying on the quality and physicochemical properties of sea cucumber under different treatment conditions at 0, 1/24, 1/4, 1, 2, 3, 4, 5 and 6 d. The study found that the soluble collagen amount increased with the prolongation of time at low-temperature treatment and the content of soluble collagen of 6 d is 3.23 times of 0 d. The hardness and chewiness dramatically decreased after 1/4 d and the hardness and chewiness are only 0.92% and 0.22% of the state of 0d. SDS-PAGE result indicated that the notable proteolysis happened to the protein with molecular weight of 200 ku and 44ku of actin with the heating time extension. Compared with the control group, the activity of cathepsin-L and caspase-3 was increased 1.6 times at 1/2 d and 3 times at 1d and their activity decreases with the prolongation of time at low-temperature treatment. ESR results indicated that the free radicals production of sea cucumber reached the highest level after 1/2 d and compared with 0 d, its value increased by 1.67 times. In summary, at 20℃ low temperature heat treatment, free radicals in sea cucumbers were generated and oxidation promotes cathepsin-L and caspase-3 activation, which leaded to degradation of protein, dissolution of collagen, and ultimately morphological and quality changes in sea cucumber. © 2019, Editorial Office of Journal of CIFST. All right reserved.
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页码：134 / 140
页数：6
相关论文
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