Evaluation of Anti-inflammatory Activity of Garlic Extracts in 3D Bioprinted Skin Equivalents

Ongoing obstacles in preclinical drug testing have raised significant concerns within the pharmaceutical industry. Recently, utilizing the potential of three-dimensional (3D) bioprinting offers a solution for creating tissue models for screening of the effectiveness and safety of new drugs. In this study, the anti-inflammatory potential of garlic extracts is assessed, specifically N-Benzyl-N-methyl-dodecan-1-amine (BMDA), using a 3D bioprinted artificial skin model. Comprehensive physicochemical characterization and immunocytochemical analysis demonstrate that the 3D bioprinted skin model exhibits structures and functions resembling human skin. BMDA treatment in a prepared skin model reveals its capacity to mitigate H2O2-induced oxidative stress and trigger anti-inflammatory responses. Notably, BMDA reduces the expression of pro-inflammatory cytokines and chemokines by downregulating NF-kappa B and mitogen-activated protein kinase inflammatory signaling pathways. In summary, our findings highlight the potential of an artificial skin model as a robust platform for the development of new drugs. This study addresses challenges in preclinical drug testing by utilizing three-dimensional (3D) bioprinted artificial skin models. The anti-inflammatory effects of garlic extract, named N-benzyl-N-methyl-dodecan-1-amine (BMDA), were tested on 3D bioprinted skin models, showing reduced oxidative stress and pro-inflammatory cytokines and chemokines expression by downregulating NF-kappa B and mitogen-activated protein kinase pathways.image (c) 2024 WILEY-VCH GmbH