Combination of the Topical Photodynamic Therapy of Chloroaluminum Phthalocyanine Liposomes with Fexinidazole Oral Self-Emulsifying System as a New Strategy for Cutaneous Leishmaniasis Treatment

被引:1
作者
Silva, Raphaela Ariany [1 ]
Damasio, Danielle Soter [1 ]
Coelho, Larissa Dutra [1 ]
de Morais-Teixeira, Eliane [2 ]
Queiroz-Junior, Celso M. [3 ]
Souza, Paulo Eduardo [4 ,5 ]
Azevedo, Ricardo Bentes [6 ]
Tedesco, Antonio [7 ]
Ferreira, Lucas Antonio [1 ]
Oliveira, Monica Cristina [1 ]
Aguiar, Marta Gontijo [1 ]
机构
[1] Univ Fed Minas Gerais, Fac Pharm, Dept Pharmaceut Prod, BR-31270901 Belo Horizonte, MG, Brazil
[2] Fundacao Oswaldo Cruz FIOCRUZ, Inst Rene Rachou, Clin Res & Publ Policy Grp Infect & Parasit Dis, BR-30190002 Belo Horizonte, MG, Brazil
[3] Univ Fed Minas Gerais, Inst Biol Sci, Dept Morphol, BR-31270901 Belo Horizonte, MG, Brazil
[4] Univ Brasilia, Inst Phys, Lab Software & Instrumentat Appl Phys, BR-70910900 Brasilia, DF, Brazil
[5] Univ Brasilia, Inst Phys, Lab Electron Paramagnet Resonance, BR-70910900 Brasilia, DF, Brazil
[6] Univ Brasilia, Inst Biol Sci, Nanobiotechnol Lab, BR-70910900 Brasilia, DF, Brazil
[7] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040900 Ribeirao Preto, Brazil
关键词
chloroaluminum phthalocyanine; liposomes; fexinidazole; self-emulsifying drug release system; cutaneous leishmaniasis; combined therapy; DRUG-DELIVERY SYSTEMS; MILTEFOSINE TREATMENT; IN-VITRO; BIOAVAILABILITY; PAROMOMYCIN;
D O I
10.3390/pharmaceutics16040509
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Cutaneous leishmaniasis (CL) is a neglected tropical disease. The treatment is restricted to drugs, such as meglumine antimoniate and amphotericin B, that exhibit toxic effects, high cost, long-term treatment, and limited efficacy. The development of new alternative therapies, including the identification of effective drugs for the topical and oral treatment of CL, is of great interest. In this sense, a combination of topical photodynamic therapy (PDT) with chloroaluminum phthalocyanine liposomes (Lip-ClAlPc) and the oral administration of a self-emulsifying drug delivery system containing fexinidazole (SEDDS-FEX) emerges as a new strategy. The aim of the present study was to prepare, characterize, and evaluate the efficacy of combined therapy with Lip-ClAlPc and SEDDS-FEX in the experimental treatment of Leishmania (Leishmania) major. Lip-ClAlPc and SEDDS-FEX were prepared, and the antileishmanial efficacy study was conducted with the following groups: 1. Lip-ClAlPc (0.05 mL); 2. SEDDS-FEX (50 mg/kg/day); 3. Lip-ClAlPc (0.05 mL)+SEDDS-FEX (50 mg/kg/day) combination; 4. FEX suspension (50 mg/kg/day); and 5. control (untreated). BALB/c mice received 10 sessions of topical Lip-ClAlPc on alternate days and 20 consecutive days of SEDDS-FEX or FEX oral suspension. Therapeutical efficacy was evaluated via the parasite burden (limiting-dilution assay), lesion size (mm), healing of the lesion, and histological analyses. Lip-ClAlPc and SEDDS-FEX presented physicochemical characteristics that are compatible with the administration routes used in the treatments. Lip-ClAlPc+SEDDS-FEX led to a significant reduction in the parasitic burden in the lesion and spleen when compared to the control group (p < 0.05) and the complete healing of the lesion in 43% of animals. The Lip-ClAlPc+SEDDS-FEX combination may be promising for the treatment of CL caused by L. major.
引用
收藏
页数:13
相关论文
共 40 条
  • [21] Liposome composition in drug delivery design, synthesis, characterization, and clinical application
    Large, Danielle E.
    Abdelmessih, Rudolf G.
    Fink, Elizabeth A.
    Auguste, Debra T.
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2021, 176
  • [22] Nanoemulsions with Chloroaluminium Phthalocyanine and Paromomycin for Combined Photodynamic and Antibiotic Therapy for Cutaneous Leishmaniasis
    Leal Pinto, Sandra Milena
    Muehlmann, Luis Alexandre
    Mantilla Ojeda, Lucia Liliana
    Vera Arias, Angelica Maria
    Roa Cordero, Martha Viviana
    Menezes Almeida Santos, Maria de Fatima
    Azevedo, Ricardo Bentes
    Escobar Rivero, Patricia
    [J]. INFECTION AND CHEMOTHERAPY, 2021, 53 (02) : 342 - 354
  • [23] Leishmaniasis, US
  • [24] Topical photodynamic therapy with chloroaluminum phthalocyanine liposomes is as effective as systemic pentavalent antimony in the treatment of experimental cutaneous leishmaniasis
    Lopes, Savia Caldeira
    Silva, Raphaela Ariany
    Novais, Marcus Vinicius
    Coelho, Larissa Dutra
    Ferreira, Lucas Antonio
    Souza, Paulo Eduardo
    Tedesco, Antonio
    Azevedo, Ricardo Bentes
    Aguiar, Marta Gontijo
    Oliveira, Monica Cristina
    [J]. PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2019, 28 : 210 - 215
  • [25] Cholesterol improves stability of amphotericin B nanoemulsion: promising use in the treatment of cutaneous leishmaniasis
    Mansur-Alves, Izabela
    Furtado Lima, Brenda Lorrayne
    Santos, Thais Tunes
    Araujo, Naialy F.
    Frezard, Frederic
    Islam, Arshad
    de Barros, Andre L. B.
    dos Santos, Delia C. M.
    Fernandes, Christian
    Ferreira, Lucas A. M.
    Aguiar, Marta M. G.
    [J]. NANOMEDICINE, 2022, 17 (18) : 1237 - 1251
  • [26] Benznidazole self-emulsifying delivery system: A novel alternative dosage form for Chagas disease treatment
    Mazzeti, Ana Lia
    Oliveira, Liliam Teixeira
    Goncalves, Karolina R.
    Schaun, Gessica C.
    Furtado Mosqueira, Vanessa Carla
    Bahia, Maria Terezinha
    [J]. EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2020, 145
  • [27] Annexin A1 Is Involved in the Resolution of Inflammatory Responses during Leishmania braziliensis Infection
    Oliveira, Leandro G.
    Souza-Testasicca, Miriam C.
    Vago, Juliana P.
    Figueiredo, Amanda Braga
    Canavaci, Adriana M. C.
    Perucci, Luiza Oliveira
    Ferreira, Tatiana P. Teixeira
    Coelho, Eduardo A. F.
    Goncalves, Denise Utsch
    Rocha, Manoel Otavio C.
    Silva, Patricia M. R. e
    Ferreira, Claudia N.
    Queiroz-Junior, Celso
    Sousa, Lirlandia P.
    Fernandes, Ana Paula
    [J]. JOURNAL OF IMMUNOLOGY, 2017, 198 (08) : 3227 - 3236
  • [28] Drug repositioning: Re-investigating existing drugs for new therapeutic indications
    Padhy, B. M.
    Gupta, Y. K.
    [J]. JOURNAL OF POSTGRADUATE MEDICINE, 2011, 57 (02) : 153 - 160
  • [29] In vitro phototoxicity of ultradeformable liposomes containing chloroaluminum phthalocyanine against New World Leishmania species
    Paola Hernandez, Indira
    Montanari, Jorge
    Valdivieso, Wilfredo
    Jose Morilla, Maria
    Lilia Romero, Eder
    Escobar, Patricia
    [J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2012, 117 : 157 - 163
  • [30] Study of the efficacy of N-methyl glucamine antimoniate (SbV) associated with photodynamic therapy using liposomal chloroaluminium phthalocyanine in the treatment of cutaneous leishmaniasis caused by Leishmania (L.) amazonensis in C57BL6 mice
    Pereira Ribeiro, Jefferson Bruno
    Miranda-Vilela, Ana Luisa
    Santarem Amorim, Ana Angelica
    Garcia, Rafaela Debastiani
    Moreira, Jonathan Rosa
    Gomes, Ciro Martins
    Soares Takano, Gustavo Henrique
    Farias de Oliveira, Gabriela Mariangela
    Lima, Alexandre Vasconcelos
    Rodrigues da Silva, Izabel Cristina
    Ribeiro Sampaio, Raimunda Nonata
    [J]. PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY, 2019, 26 : 261 - 269