Drug resistance and genetic characteristics of one Eimeria tenella isolate from Xiantao, Hubei Province, China

被引：2

作者：

Geng, Tiantian ^{[1
]}

Luo, Liyan ^{[1
]}

Ruan, Xiaodie ^{[1
]}

Shen, Bang ^{[1
,2
]}

Fang, Rui ^{[2
]}

Zhao, Junlong ^{[1
,2
,3
]}

Zhou, Yanqin ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Coll Vet Med, Key Lab Prevent Vet Hubei Prov, Wuhan 430070, Hubei, Peoples R China

[2] Huazhong Agr Univ, State Key Lab Agr Microbiol, Shizishan St 1, Wuhan 430070, Hubei, Peoples R China

[3] Shanchuan Biotechnol Wuhan Co Ltd, Wuhan, Peoples R China

来源：

PARASITOLOGY RESEARCH | 2024年 / 123卷 / 08期

关键词：

Eimeria tenella; Broiler; ITS-1; Whole genome resequencing; Drug resistance; ANTICOCCIDIAL DRUGS; DNA VACCINE; COCCIDIOSIS; IDENTIFICATION; EFFICACY;

D O I：

10.1007/s00436-024-08310-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Chicken coccidiosis causes retarded growth and low production performance in poultry, resulting in huge economic losses to the poultry industry. In order to prevent and control chicken coccidiosis, great efforts have been made to develop new drugs and vaccines, which require pure isolates of Eimeria spp. In this study, we obtained the Eimeira tenella Xiantao isolate by single oocyst isolation technology and compared its genome with the reference genome GCF_000499545.2_ETH001 of the Houghton strain. The results of the comparative genomic analysis indicated that the genome of this isolate contained 46,888 single nucleotide polymorphisms (SNPs). There were 15,107 small insertion and deletion variations (indels), 1693 structural variations (SV), and 3578 copy number variations (CNV). In addition, 64 broilers were used to determine the resistance profile of Xiantao strain. Drug susceptibility testing revealed that this isolate was completely resistant to monensin, diclazuril, halofuginone, sulfachlorpyrazine sodium, and toltrazuril, but sensitive to decoquinate. These data improve our understanding of drug resistance in avian coccidia.

引用

页数：12

共 42 条

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