Alkaloid-metabolites from the mangrove-derived fungus Penicillium robsamsonii HNNU0006

Nine metabolites, including three undescribed alkaloids pyripyropenes V-W (1-2), penicioxa A (4), two previously reported pyripyropene A (3), oxaline (5), three grisephenone-type xanthone derivatives (6-8), and a diphenyl ether derivative 4-chloro-7,4 '-dihydroxy-5,2 '-dimethoxy-2-methylformate-6 '-methybenzophone (9), were isolated from cultures of the mangrove-derived fungus Penicillium robsamsonii HNNU0006. Their structures were determined by spectroscopic analysis, ECD calculations, together with DP4+ probability analysis. Furthermore, all the isolated compounds were tested for cytotoxicity and anti-phytopathogenic fungal activities. Compounds 6-8 showed moderate cytotoxicity against tumor cell lines A549, with IC50 values ranging from 5.68 +/- 0.21 to 9.71 +/- 0.34 mu g/mL, respectively.