Delivery of Experimental mRNA Vaccine Encoding the RBD of SARS-CoV-2 by Jet Injection

被引：1
作者：
Kisakov, D. N. [1 ]
Kisakova, L. A. [1 ]
Sharabrin, S. V. [1 ]
Yakovlev, V. A. [1 ]
Tigeeva, E. V. [1 ]
Borgoyakova, M. B. [1 ]
Starostina, E. V. [1 ]
Zaikovskaya, A. V. [1 ]
Rudometov, A. P. [1 ]
Rudometova, N. B. [1 ]
Karpenko, L. I. [1 ]
Ilyichev, A. A. [1 ]
机构：
[1] State Res Ctr Virol & Biotechnol VECTOR, Fed Serv Surveillance Consumer Rights Protect & Hu, Koltsov, Novosibirsk Reg, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2024年 / 176卷 / 06期
关键词：
mRNA vaccines; jet injection; delivery methods; receptor-binding domain of the S protein of the SARS-CoV-2 virus;
D O I：
10.1007/s10517-024-06107-x
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
We studied a needle-free jet injection delivery of an experimental mRNA vaccine encoding the receptor-binding domain of the SARS-CoV-2 S protein (mRNA-RBD). Immunization of BALB/c mice with mRNA-RBD by a needle-free jet injector induced high levels of antibodies with virus-neutralizing activity and a virus-specific T-cell response. The immune response was low in the group of mice that received intramuscular injection of mRNA-RBD. The effectiveness of this simple and safe method of mRNA delivering has been demonstrated. Thus, jet injection of mRNA vaccine can be a good alternative to lipid nanoparticles.
引用
收藏
页码：776 / 780
页数：5
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