Structural Analysis and Phylogenetic Relationships of a Teleost Fish, Pethia stoliczkana Based on the Complete Mitochondrial Genome Sequence

In this study, the whole mitochondrial genome sequence of Pethia stoliczkana was obtained using highthroughput sequencing technology, and its structure and characteristics were analyzed. The P. stoliczkana mitochondrial genome contained a total of 16,966 base pairs, including 13 protein-coding genes, 22 transport RNA genes, two ribosomal RNA genes, and one control region. The A+T content (59.7%) of the whole mitochondrial genome was greater than the G+C content (40.3%), indicating an obvious A+T preference. The mitochondrial genome of P. stoliczkana is similar to that of most teleost fish, and no gene rearrangements were detected. The phylogenetic relationship tree of Smiliogastrinae fish was constructed based on 13 protein-coding genes using the Bayesian inference and maximum likelihood methods. We found that P. stoliczkana was closely related to Pethia ticto and Pethia padamya. These results enrich the mitochondrial genome database of Smiliogastrinae fish and provide reference materials for systematic classification of this group of fish.