A novel and highly selective aptamer-based sandwich-type biosensor assisted by second-order calibration methods for efficient biosensing of Streptococcus pneumoniae

被引：0

作者：

Yaghoobi A. ^{[1
]}

Abiri R. ^{[2
]}

Alvandi A. ^{[2
]}

Arkan E. ^{[3
]}

Jalalvand A.R. ^{[4
]}

机构：

[1] Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah

[2] Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah

[3] Nano Drug Delivery Research Center, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah

[4] Research Center of Oils and Fats, Research Institute for Health Technology, Kermanshah University of Medical Sciences, Kermanshah

来源：

Sensors International | 2022年 / 3卷

关键词：

Sandwich-type biosensor; Second-order calibration; Streptococcus pneumoniae; Trouble shooting;

D O I：

10.1016/j.sintl.2022.100203

中图分类号：

学科分类号：

摘要：

In this article, a novel electrochemical biosensor was fabricated based on modification of a screen printed carbon electrode (SPCE) with 5′-GTT GGAATG CTG AGA AAA AAA AAA-Thiol-C3-3' (CAP)/lead nanoparticles/fullerene C60-multiwalled carbon nanotubes with the aim of biosensing of the Streptococcus pneumoniae (SPP). Single stranded DNAs extracted from the SPPs were used as targets (TAGs) which were captured by the CAPs, and by 5′-6-Fam-AAAAAC GGT TGAACT GAT TG-3' (APT) formed an APT-TAG-CAP sandwich assay for the detection of single stranded DNAs of the SPP. The differential pulse voltammetric (DPV) response of the biosensor immersed into the electrochemical probe solution was changed upon formation of the APT-TAG-CAP sandwich assay which was used to the determination of the number of the SPPs. In order to increase the selectivity of the biosensor towards determination of the number of the SPPs, it was assisted by second-order calibration methods based on generation of second-order DPV data, data pre-processing, trouble shooting, and data analysis by U-PLS/RBL (sensitivity: 22 μA (ng/μL)-1 single stranded DNA/22 μA (283 SPPs)-1 and LOD: 0.1 ng/μL single stranded DNA/28 SPPs) and N-PLS/RBL as well-known second-order algorithms. The biosensor assisted by U-PLS/RBL showed a better performance towards determination of the SPP whose performance was comparable with the NanoDrop method as reference method. © 2022 The Authors

引用

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