Creation of Bacterial Blight Resistant Rice by Targeting Homologous Sequences of Xa13 and Xa25 Genes

被引：3

作者：

Zhu, Yiwang ^{[1
,2
]}

Yang, Xiaohuai ^{[3
]}

Luo, Peirun ^{[3
]}

Yan, Jingwan ^{[1
]}

Cao, Xinglan ^{[2
,4
]}

Qian, Hongge ^{[2
]}

Zhu, Xiying ^{[2
,4
]}

Fan, Yujin ^{[2
,4
]}

Mei, Fating ^{[1
]}

Fan, Meiying ^{[1
]}

Shang, Lianguang ^{[2
]}

Wang, Feng ^{[1
]}

Zhang, Yu ^{[5
]}

机构：

[1] Fujian Acad Agr Sci, Inst Biotechnol, Fujian Prov Key Lab Genet Engn Agr, Fuzhou 350003, Peoples R China

[2] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Genome Anal Lab Minist Agr & Rural Affairs,Guangdo, Shenzhen 518124, Peoples R China

[3] Shenzhen Agr Sci & Technol Promot Ctr, Shenzhen 518000, Peoples R China

[4] Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China

[5] Food Crops Res Inst, Yunnan Acad Agr Sci YAAS, Yunnan Seed Lab, Yunnan Key Lab Rice Genet Improvement, Kunming 650200, Peoples R China

来源：

AGRONOMY-BASEL | 2024年 / 14卷 / 04期

关键词：

bacterial blight resistant; CRISPR/Cas9; Xa13; Xa25; rice; PLANTS; CRISPR/CAS9; XA5;

D O I：

10.3390/agronomy14040800

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Bacterial blight is a destructive disease in rice caused by Xanthomonas oryzae pv. oryzae (Xoo). Single resistance genes often have limitations in providing broad-spectrum resistance, as pathogens continuously evolve and vary. Breeding rice varieties with multiple disease resistance genes has proven to be an effective strategy for controlling bacterial blight. In this study, a single Cas9/gRNA construct was used to target the homologous sequences of Xa13 and Xa25 genes through destroying the target gene function, creating bacterial blight resistance in five rice varieties. These materials provide promising germplasm resources for the development of rice varieties with durable resistance to bacterial blight.

引用

页数：9

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