Molecularly imprinted conducting polymer based sensor for Salmonella typhimurium detection

被引：5
作者：
Aparna [1 ]
Garg M. [1 ]
Vishwakarma N. [1 ]
Mizaikoff B. [2 ,3 ]
Singh S. [1 ]
机构：
[1] CSIR-Central Scientific Instruments Organisation (CSIR-CSIO), Chandigarh
[2] Institute of Analytical and Bioanalytical Chemistry, Ulm University
[3] Hahn-Schickard Institute for Microanalysis Systems, Ulm
来源：
Bioelectrochemistry | 2022年 / 147卷
关键词：
Biomimetic Sensor; Electrochemical Sensor; Molecularly Imprinted Conducting Polymer; Salmonella typhimurium;
D O I：
10.1016/j.bioelechem.2022.108211
中图分类号：
学科分类号：
摘要：
This manuscript reports the design and fabrication of conducting plastibody based electrochemical sensor for the detection of Salmonella typhimurium. The conductive plastibody was fabricated on an Indium Tin Oxide surface through potentiostatic method (electrodeposition for 400 s), wherein a polymer mix of pyrrole, lactic acid, ammonium chloride, and sodium dodecyl sulfate was employed for the electrodeposition. Various template removal methods were tested and electrochemical cleaning in the MES buffer was found to be the most suitable, which was optimized further. The synthesized plastibody sensors were characterized using electrochemical impedance spectroscopy, contact angle, FTIR spectroscopy and scanning electron microscopy. Amperometry was used as the electrochemical analytical technique for the determination of the analyte in the concentration range of 100 –108 CFU/mL having a limit of detection of 3.42 CFU/mL. Sensor's performance was also compared with the non-imprinted electrode and an imprinting factor of 3.8 was found. The plastibody sensor was tested against other bacteria and coefficient of selectivity was calculated to be 1.0, 10.8, 5.6 and 2.4 towards S. typhi, S. aureus, E. coli and L. monocytogenes respectively. The sensor was also found to be reproducible in nature (RSD 0.11 %) and this generic concept presented herein may be extended for the detection of pathogens in other matrices as well. © 2022 Elsevier B.V.
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共 37 条
[1]  
Newell D.G., Koopmans M., Verhoef L., Duizer E., Aidara-Kane A., Sprong H., Opsteegh M., Langelaar M., Threfall J., Scheutz F., der Giessen J.V., Kruse H., Food-borne diseases — The challenges of 20years ago still persist while new ones continue to emerge, Int. J. Food Microbiol., 139, pp. S3-S15, (2010)
[2]  
Leonard P., Hearty S., Brennan J., Dunne L., Quinn J., Chakraborty T., O'Kennedy R., Advances in biosensors for detection of pathogens in food and water, Enzyme Microb. Technol., 32, 1, pp. 3-13, (2003)
[3]  
Mahony J.O., Nolan K., Smyth M.R., Mizaikoff B., Molecularly imprinted polymers—potential and challenges in analytical chemistry, Anal. Chim. Acta, 534, 1, pp. 31-39, (2005)
[4]  
Lowdon J.W., Dilien H., Singla P., Peeters M., Cleij T.J., van Grinsven B., Eersels K., MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo, Sens. Actuators, B, 325, (2020)
[5]  
Reddy S.M., Hawkins D.M., Phan Q.T., Stevenson D., Warriner K., Protein detection using hydrogel-based molecularly imprinted polymers integrated with dual polarisation interferometry, Sens. Actuators, B, 176, pp. 190-197, (2013)
[6]  
Idil N., Mattiasson B., Imprinting of Microorganisms for Biosensor Applications, Sensors (Basel, Switzerland), 17, 4, (2017)
[7]  
Jamalipour Soufi G., Iravani S., Varma R.S., Molecularly imprinted polymers for the detection of viruses: challenges and opportunities [10.1039/D1AN00149C], Analyst, 146, 10, pp. 3087-3100, (2021)
[8]  
Jia M., Zhang Z., Li J., Ma X., Chen L., Yang X., Molecular imprinting technology for microorganism analysis, TrAC, Trends Anal. Chem., 106, pp. 190-201, (2018)
[9]  
Svenson J., Nicholls I.A., On the thermal and chemical stability of molecularly imprinted polymers, Anal. Chim. Acta, 435, 1, pp. 19-24, (2001)
[10]  
Latif U., Can S., Sussitz H.F., Dickert F.L., Molecular Imprinted Based Quartz Crystal Microbalance Sensors for Bacteria and Spores, Chemosensors, 8, 3, (2020)
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