A Fluorescent Sensing System Based on Genefinder and Aptamer for the Detection of Ochratoxin A

被引：0
|
作者：
Guo T. [1 ]
Ye Z. [1 ]
Zhou H. [1 ]
Zhang Y. [1 ,2 ]
Ma L. [1 ,2 ]
机构：
[1] College of Food Science, Southwest University, Chongqing
[2] Biological Science Research Center, Southwest University, Chongqing
来源：
Shipin Kexue/Food Science | 2021年 / 42卷 / 20期
关键词：
Aptamer; Fluorescence sensing system; Genefinder; Ochratoxin A;
D O I：
10.7506/spkx1002-6630-20201016-144
中图分类号：
学科分类号：
摘要：
A fluorescence sensing system based on the nucleic acid dye Genefinder and aptamer was constructed for the detection of ochratoxin A (OTA) in this study. A double-stranded structure was formed by binding the OTA aptamer to its complementary strand. Binding of Genefinder to double-stranded DNA (dsDNA) generated a strong fluorescent signal. In the presence of OTA, the aptamer bound to it to form a quadruplex structure, melting the double strands and leading to a decrease of the fluorescence intensity of Genefinder. The results showed that the linear range of the sensing system was 50 pg/L-300 ng/L, and the detection limit was 50 pg/L. The sensing system had a high selectivity for OTA and could be widely applied as a simple and fast method. © 2021, China Food Publishing Company. All right reserved.
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页码：305 / 309
页数：4
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