Cloning of a Novel Xylanase Gene cbxynA Based on Macro Gene Strategy and the Characterization of Recombinant Enzyme

被引：0

作者：

Xiong K. ^{[1
,2
]}

Cui X. ^{[1
,2
]}

Wang X. ^{[1
,4
]}

Zhao Z. ^{[1
,3
]}

Liu J. ^{[2
,4
]}

Pei P. ^{[2
,3
]}

Deng L. ^{[3
,4
]}

Xiong S. ^{[1
,2
]}

机构：

[1] Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, Beijing

[2] Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing

[3] Beijing Laboratory for Food Quality and Safety, Beijing Technology & Business University, Beijing

[4] Beijing Key Laboratory of Flavor Chemistry, Beijing Technology & Business University, Beijing

来源：

Journal of Chinese Institute of Food Science and Technology | 2019年 / 19卷 / 09期

关键词：

Enzymatic properties; Metagenomic library; Soil microbe; Xylanase;

D O I：

10.16429/j.1009-7848.2019.09.004

中图分类号：

学科分类号：

摘要：

This research construct the soil metagenomic library, which have 1.3×104 storage capacity and 30 Mb length of the total gene. Screening the positive clone can hydrolyze xylan from the library based on the functional strategy. Sequence analysis revealed that cbxynA potential encoding xylanase gene with 822 bp length. Only 45% of its amino acid sequence similar with the encoding xylanase from Halobacteriaceae archaeon(Archaea), which indicated that it's a novel unknown sequence. Molecular weight of cbxynA expressed in the E. coli was 29 ku consistented with predicted size. At the same time, the prokaryotic expression conditions were optimized. The results showed that the plasmid cultivated for 5 h, then added 0.7 mmol/L IPTG at 23℃ inducing for 25 h, and the xylanase activity was 52 U/mL. The optimum pH and temperature of the recombinant enzyme CBXYNA were 5.2 and 55℃, respectively. The metal ions of Na+, K+, Li+ and Ca2+ can enhance the enzyme activity. While Mg2+, Mn2+, Zn2+, Fe3+, Al3+ and Cu2+ can inhibit the activity of the enzyme. When CBXYNA taken water soluble corncob xylan and oat xylan, water-insoluble corncob xylan as substrate, the relative enzyme activity was 162%, 139%, 74%, respectively. © 2019, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：37 / 44

页数：7

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