Antimicrobial Peptides Screened from Penaeus vannamei Shrimp and Investigation of Their DNA Binding Mechanism

被引：0
作者：
Wang N. [1 ]
Zheng N. [1 ,2 ,3 ]
Ren Z. [1 ,2 ,3 ]
Shi L. [1 ,2 ,3 ]
Deng S. [4 ]
Yang S. [1 ,2 ,3 ]
机构：
[1] College of Ocean Food and Biological Engineering, Jimei University, Xiamen, Fujian
[2] Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, Fujian
[3] Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Liaoning, Dalian
[4] Zhejiang Xingye Group Co., Ltd., Zhejiang, Zhoushan
来源：
Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 07期
关键词：
antimicrobial activity; antimicrobial peptide; DNA binding mechanism; Penaeus vannamei; Vibrio parahaemolyticus;
D O I：
10.16429/j.1009-7848.2023.07.015
中图分类号：
学科分类号：
摘要：
The antimicrobial activity and DNA binding mechanism of a novel antimicrobial peptide (ANP) PV13 against Vibrio parahaemolyticus from Penaeus vannamei were explored. The sequence of the peptides were identified by using ultra -high -performance liquid chromatography -high resolution mass spectrometry, and the cationic antimicrobial peptide PV13 (ALPWVLPWALPRALPRVLPR) was screened by using in silico tools. The antimicrobial activity of ANP PV13 against V. parahaemolyticus was evaluated by minimum inhibitory concentration (NIC) and time-kill curve, the NIC of PV13 was 62.5 μg/mL and killed V. parahaemolyticus within 2 h. The antibacterial mechanism of PV13 against V. parahaemolyticus was evaluated using transmission electron microscopy (TEN), intracellular membrane permeability, DNA gel retardation and circular dichroism. The results shows that PV13 increases bacterial cell membrane permeability and binds bacteria genomic DNA concentration dependently. Noreover, ANP PV13 exhibited a random coil structure in PBS and SDS solutions, while its structure changed significantly after binding with DNA of V. parahaemolyticus. The antimicrobial activity of peptide PV13 could be due to the presence of four leucine-proline repeat regions. The above results provide new insight into screening and designing ANPs from P. vannamei, and offer a theoretical basis of ANP PV13 for their potential applications in food preservation. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
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页码：140 / 151
页数：11
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