Aerobic Anoxygenic Phototrophic Bacteria in the Marine Environments Revealed by Raman/Fluorescence-Guided Single-Cell Sorting and Targeted Metagenomics

被引：1

作者：

Xu, Lin ^{[1
,2
,3
]}

Yue, Xiao-Lan ^{[1
,2
,4
]}

Li, Hong-Zhe ^{[5
]}

Jian, Shu-Ling ^{[1
,2
,5
]}

Shu, Wen-Sheng ^{[6
]}

Cui, Li ^{[5
]}

Xu, Xue-Wei ^{[1
,2
,4
]}

机构：

[1] Minist Nat Resources, Key Lab Marine Ecosyst Dynam, Hangzhou 310012, Peoples R China

[2] Minist Nat Resources, Inst Oceanog 2, Hangzhou 310012, Peoples R China

[3] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Oceanog, Shanghai 200030, Peoples R China

[5] Chinese Acad Sci, Key Lab Urban Environm & Hlth, Inst Urban Environm, Xiamen 361021, Peoples R China

[6] South China Normal Univ, Inst Ecol Sci, Sch Life Sci, Guangzhou 510631, Peoples R China

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 2024年 / 58卷 / 16期

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

single-cell microbiology; Raman/fluorescence spectroscopy; aerobic anoxygenic phototrophic bacteria; bacteriochlorophylla; pelagic environments; Sphingomonadales; RHODOBACTER-SPHAEROIDES; RAMAN-SPECTROSCOPY; ALGORITHM; SOFTWARE; GENOMICS; PROTEIN; GROWTH; GENES; LIGHT; HEME;

D O I：

10.1021/acs.est.4c02881

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Aerobic anoxygenic phototrophic bacteria (AAPB) contribute profoundly to the global carbon cycle. However, most AAPB in marine environments are uncultured and at low abundance, hampering the recognition of their functions and molecular mechanisms. In this study, we developed a new culture-independent method to identify and sort AAPB using single-cell Raman/fluorescence spectroscopy. Characteristic Raman and fluorescent bands specific to bacteriochlorophyll a (Bchl a) in AAPB were determined by comparing multiple known AAPB with non-AAPB isolates. Using these spectroscopic biomarkers, AAPB in coastal seawater, pelagic seawater, and hydrothermal sediment samples were screened, sorted, and sequenced. 16S rRNA gene analysis and functional gene annotations of sorted cells revealed novel AAPB members and functional genes, including one species belonging to the genus Sphingomonas, two genera affiliated to classes Betaproteobacteria and Gammaproteobacteria, and function genes bchCDIX, pucC2, and pufL related to Bchl a biosynthesis and photosynthetic reaction center assembly. Metagenome-assembled genomes (MAGs) of sorted cells from pelagic seawater and deep-sea hydrothermal sediment belonged to Erythrobacter sanguineus that was considered as an AAPB and genus Sphingomonas, respectively. Moreover, multiple photosynthesis-related genes were annotated in both MAGs, and comparative genomic analysis revealed several exclusive genes involved in amino acid and inorganic ion metabolism and transport. This study employed a new single-cell spectroscopy method to detect AAPB, not only broadening the taxonomic and genetic contents of AAPB in marine environments but also revealing their genetic mechanisms at the single-genomic level.

引用

页码：7087 / 7098

页数：12