A rapid and simple procedure for the whole-mount bone staining of small fish

被引：0

作者：

Yeesan, Narawadee ^{[1
]}

Sudtongkong, Chanyut ^{[1
]}

Kong-Oh, Supparat ^{[1
]}

Angsujinda, Kitipong ^{[2
]}

Kettratad, Jes ^{[3
]}

Poolprasert, Pisit ^{[4
]}

Iida, Atsuo ^{[5
]}

Boonyoung, Piyakorn ^{[6
]}

Nganvongpanit, Korakot ^{[7
]}

Kenthao, Anan ^{[8
]}

Kaneko, Gen ^{[9
]}

Senarat, Sinlapachai ^{[1
,10
]}

机构：

[1] Rajamangala Univ Technol Srivijaya, Fac Sci & Fisheries Technol, Dept Marine Sci & Environm, Trang, Thailand

[2] Chulalongkorn Univ, Aquat Resources Res Inst, Bangkok, Thailand

[3] Chulalongkorn Univ, Fac Sci, Dept Marine Sci, Bangkok, Thailand

[4] Kasetsart Univ, Fac Agr, Dept Entomol, Bangkok, Thailand

[5] Nagoya Univ, Grad Sch Bioagr Sci, Dept Anim Sci, Nagoya, Japan

[6] Prince Songkla Univ, Fac Sci, Div Hlth & Appl Sci, Hat Yai, Thailand

[7] Chiang Mai Univ, Fac Vet Med, Res Ctr Vet Biosci & Vet Publ Hlth, Chiang Mai, Thailand

[8] Naresuan Univ, Fac Sci, Dept Biol, Phitsanulok, Thailand

[9] Univ Houston Victoria, Coll Nat & Appl & Sci, Victoria, TX USA

[10] Prince Songkla Univ, Fac Sci, Div Biol Sci, Hat Yai, Thailand

来源：

JOURNAL OF HISTOTECHNOLOGY | 2024年 / 47卷 / 04期

关键词：

Skeleton system; small estuarine fish; Thailand; whole-mount bone staining; CARTILAGE; PROTOCOL;

D O I：

10.1080/01478885.2024.2339452

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The cartilage and bone structure has provided insightful knowledge about evolution and ecology of fish, which is an important component of biological oceanography. However, the whole-body bone staining is a lengthy and complicated process that typically takes five days to several months, and the improvement of the conventional method has been one of the important issues in this field. Here we report a quick and easy whole-mount bone staining method for small fish, in which a newly designed fixative is applied. Compared to conventional methods, this novel protocol is a straightforward process that could be adopted for small estuarine fish and other small vertebrates.

引用

页码：166 / 172

页数：7

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